【产品介绍】AIF, o r apoptosis-inducing fact o r , is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, o r apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced a n d causes mitochondria to release the apoptogenic proteins, cytochrome c a n d caspase-9. Apoptotic activity of AIF Rabbit Antibody,Rabbit Monoclonal AIF leads to chromatin condensation a n d DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxid o r eductase function a n d a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4). AIF Rabbit Antibody,Rabbit Monoclonal WB: 1:1,000 IHC: 1:500 ICC: 1:500 Flow Cytometry: 1:50 IP: 1:10 AIF Rabbit Antibody,Rabbit Monoclonal A. Western blot analysis of K562 cell lysate using anti-AIF RabMAb (catalog #1020-1), 1:1,000 dilution. B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-AIF RabMAb (catalog #1020-1). C. Immunoflu o r escent staining of HeLa cells, using AIF RabMAb. (Green flu o r escence- AIF localized in mitochondria, Red - PI nuclear staining). (catalog #1020-1). AIF Rabbit Antibody,Rabbit Monoclonal D. Flow cytometric analysis of permeabilized K562 cells using anti-AIF RabMAb (red) (catalog #1020-1) o r a rabbit IgG (negative)(green). |