先使微生物在极低温度(-70℃左右)下快速冷冻,然后在减压下利用升华现象除去水分(真空干燥)。有些方法如滤纸保藏法、液氮保藏法和冷冻干燥保藏法等均需使用保护剂来制备细胞悬液,以防止因冷冻或水分不断升华对细胞的损害。保护性溶质可通过氢和离子键对水和细胞所产生的亲和力来稳定细胞成分的构型。保护剂有牛乳、血清、糖类、甘油、二甲亚砜等。
OAC 10490 [CBS 506.66]Microascus manginii Loubiere
FS-30 [ARP 074]Agaricus pattersonae Peck, teleomorph
XO 7Tetrahymena sonneborni Nyberg
pBendBluefreeze-dried
Pseudomonas sp.freeze-dried
YJR152W BY4743, heterozygous diploid [22630]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
UCPCryptosporidium parvum Tyzzer
KETRI 269Trypanosoma brucei rhodesiense Stephens and ..
Clonal Human Adipose-derived stem cells: PL ..frozen
Hs 184.THomo sapiens, human bone
P1253 [22109]Phytophthora megasperma f. sp.medicagini ..
Tick cell line, IDE8frozen
YDR283C BY4743, heterozygous diploid [23642]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
YPL046C BY4743, heterozygous diploid [22782]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
HUT 4104Byssochlamys nivea Westling
74Cercospora oryzae Miyake, anamorph
YBL012C BY4742, mating type alpha [13038]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
pLW45Homo sapiens, human
POVCA1 PLASMID DNAfrozen
YGR157W BY4741 [4787]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
Toronto-3 [NCTC 11975]Fluoribacter bozemanae (Brenner et al.) Gar ..
YAL064C-A BY4743, heterozygous diploid [2571 ..Saccharomyces cerevisiae Meyen ex E.C. Hans ..
Steinernema diaprepesi (Nematode): S. diapre ..frozen
Genomic DNA from Rhodobacter sphaeroides str ..Rhodobacter sphaeroides (van Niel) Imhoff e ..
p8Homo sapiens, human
Plasmid, hKGF/pCEV9/CLone32frozen
pSE1000Homo sapiens, human
异常汉逊酵母变种使用说明书freeze-dried
YDR419W BY4742, mating type alpha [14255]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
3 [Garcia strain]Corynebacterium urealyticum Pitcher et al.
YNL190W BY4741, mating type a [2022]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
KD1Homo sapiens, human
UN/Mcb/M185Nocardia otitidiscaviarum Snijders
Sy-IH4Gibberella fujikuroi (Sawada) Wollenweber, ..
Sugarcane mosaic virusfreeze-dried
Banzi virusfreeze-dried
409-AYarrowia lipolytica (Wickerham et al.) van ..
NRS 1198Lysinibacillus sphaericus
YPL033C BY4743, heterozygous diploid [22795]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
Jan-99Tremella fuciformis Berkeley
NMR1-6Aeromonas jandaei Carnahan et al.
YGR087C BY4743, homozygous diploid [34717]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
W [CBS 234.73]Nodulisporium tuberum Fontana et Bonfante e ..
MTF0951Mus musculus, mouse
YIp5-32phocatfreeze-dried
yWXD5435Homo sapiens, human
1040Epichloe brachyelytri Schardl et Leuchtmann
YOR304W BY4743, heterozygous diploid [21601]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
Soybean Seed S080098dried
YNL088W BY4743, heterozygous diploid [22988]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
M1027 [NCTC 10025]Neisseria meningitidis (Albrecht and Ghon) ..
YBR172C BY4743, homozygous diploid [33312]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
1908Moraxella catarrhalis (Frosch and Kolle) Bo ..
NIH 10 [593]Pseudomonas aeruginosa (Schroeter) Migula
CBS 384.32Verticillium lecanii (Zimmermann) Viegas, a ..
769.2.8Cochliobolus heterostrophus Drechsler, tele ..
ALC6Rattus norvegicus, rat
Eph4 1424.2Mus musculus breast; derived from metastati ..
pILMALB5Homo sapiens, human
MC-94 [EG30]Saccharomyces cerevisiae Meyen ex E.C. Hans ..
YBR056W BY4743, heterozygous diploid [23193]Saccharomyces cerevisiae Meyen ex E.C. Hans ..