1)贴壁细胞传代:提前将培养基、PBS放入37℃水浴锅内预热,用75%酒精擦拭后再放入超净台内,吸除或倒掉细胞瓶内旧培养液,加少量PBS润洗细胞,加入适量胰酶,使胰酶的量能盖住细胞,37℃孵育,每隔2~3min显微镜下观察,待贴壁细胞间间隙变大、细胞趋于圆形但还未漂起时弃去胰酶,加入新鲜培养基,晃动细胞瓶,终止胰酶作用,用吸管小心吹打贴壁的细胞,制成细胞悬液。控制吹打的力度,避免产生大量的气泡,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养,隔天观察贴壁生长情况。
2)悬浮细胞传代:将细胞悬液转移到无菌离心管内,1000rpm离心5min,弃去上清,加入新鲜的培养基,用吸管小心吹散沉淀,制成细胞悬液,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养。
1. 收到细胞后,若发现干冰已挥发干净、冻存管瓶盖脱落、破损及细胞有污染,请立即与我们联系。
2. 所有动物细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护,所有废液及接触过此细胞的器皿需要灭菌后方能丢弃。
用于菌落总数,大肠菌群,金葡菌,大肠杆菌样品制备Histamine producing bacteria Medium
用于菌落总数,大肠菌群,金葡菌,大肠杆菌样品制备250gHistamine producing bacteria Medium
用于菌落总数测定Honda
用于菌落总数测定。250gHonda Toxin-Producing Broth
用于菌种保存(瓷珠法)Hottinger's Agar
用于菌种保存(瓷珠法)250gHottinger's Agar
用于抗生素敏感试验Hydrogen Sulfide Indole Motility Medium
用于抗生素敏感试验250gH-顶层培养基
用于抗生素效价测定Incomplete Agar Medium
用于抗生素效价测定的底层培养基或菌种活化250gIndia ink
用于空肠弯曲杆菌的选择性增菌Indole Medium
用于空肠弯曲菌的分离培养250gInorganic Agar
用于空肠弯曲菌的检测。Inorganic phosphorus bacteria
用于空肠弯曲菌的前增菌250gInorganic Salt Medium
用于空肠弯曲菌的增菌(GB标准)Intergrated Potato Medium
用于空肠弯曲菌的增菌培养250gIodine solution
用于空肠弯曲菌过氧化氢酶试验Iron Agar
用于空肠弯曲菌马尿酸钠水解试验250gIron Agar
用于空肠弯曲菌选择性增菌培养。Iron bacteria medium
C15H26OPregn-5-en-20-one,3-[(O-2,6-dideoxy-3-O-methyl-b-D-arabino-hexopyranosyl-(14)-O-2,6-dideoxy-3-O-methyl-b-D-ribo-hexopyranosyl-(14)-2,6-dideoxy-3-O-methyl-b-D-ribo-hexopyranosyl)oxy]-8,14,17-trihydroxy-12-[(4-hydroxybenzoyl)oxy]-,(3b,12b,14b,17a)- (9CI)甜菊苷929.09554
结膜上皮细胞上海直销C15H26OPregnenolone通关藤苷G933.1273
C15H26N2.H2O4S.5[H2O]Presapogenin CP4通关藤苷H934
C15H26N2.H2O4S.5[H2O]prim-O-Glucosylcimifugin通关藤苷I943.12212
C15H24O5Proanthocyanidin B1通关藤苷X947.14
C15H24O2Procyanidin土贝母苷甲947.15
C15H24O2Protodioscin土槿皮丙酸947.158
C15H24O10Protopanaxatriol土槿皮甲酸951.0134
C15H24N2O2Protopanaxatriol土槿皮甲酸-葡萄糖苷952.64482
C15H24N2OPROTOPANAXDIOL土槿皮乙酸-葡萄糖苷959.12
C15H24N2OPROTOPANAXDIOL土荆皮乙酸;土荆乙酸; 土槿皮乙酸959.12152
C15H22O9protopine土木香内酯963.15
C15H22O5Pseudoginsenoside F11蜕皮激素963.15
C15H22O5Pseudoginsenoside Rh2脱氢紫堇碱963.15328
C15H22O3Pseudohypericin脱水穿心莲内酯967.01
C15H22O2Pseudolaric Acid A;2,4-Pentadienoic acid, 5-(4a-(acetyloxy)-3,4,4a,5,6,9-hexahydro-3,7-dimethyl-1-oxo-1H-4,9a-ethanocyclohepta(c)pyran-3-yl)-2-methyl-(3alpha(2E,4E),4alpha,4aalpha,9aalpha)-(-)-脱水长春碱975.12092
C15H22O10Pseudolaric acid B脱氧野尻霉素976.96
C15H22OPseudolaric Acid C脱乙酰灵芝酸F976.96
C15H22OPseudoprotodioscin王不留行黄酮苷1031.18
C15H22Opsoralen伪金丝桃素1031.18418
C15H22N2O2Pterostilbene伪原薯蓣皂苷1045.21