1)贴壁细胞传代:提前将培养基、PBS放入37℃水浴锅内预热,用75%酒精擦拭后再放入超净台内,吸除或倒掉细胞瓶内旧培养液,加少量PBS润洗细胞,加入适量胰酶,使胰酶的量能盖住细胞,37℃孵育,每隔2~3min显微镜下观察,待贴壁细胞间间隙变大、细胞趋于圆形但还未漂起时弃去胰酶,加入新鲜培养基,晃动细胞瓶,终止胰酶作用,用吸管小心吹打贴壁的细胞,制成细胞悬液。控制吹打的力度,避免产生大量的气泡,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养,隔天观察贴壁生长情况。
2)悬浮细胞传代:将细胞悬液转移到无菌离心管内,1000rpm离心5min,弃去上清,加入新鲜的培养基,用吸管小心吹散沉淀,制成细胞悬液,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养。
1. 收到细胞后,若发现干冰已挥发干净、冻存管瓶盖脱落、破损及细胞有污染,请立即与我们联系。
2. 所有动物细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护,所有废液及接触过此细胞的器皿需要灭菌后方能丢弃。
用于培养营养要求高的微生物Maconkey Agar No.3
用于培养支原体的基础培养基250gMaconkey Agar(without salt)
用于培养支原体的基础培养基Malonate Broth
用于配制血琼脂、巧克力琼脂及亚碲酸盐琼脂250gMalt Extract Agar
用于皮肤癣菌鉴别培养Malt Extract Agar
用于啤酒和发酵产品中酵母菌和细菌的计数250gMalt Extract Broth
用于啤酒检验Malt Extract Medium
用于啤酒中乳酸菌检测和分离培养4ml*2支/盒Manitol Salt Agar
用于啤酒中厌氧菌检测250gMannitol Medium
用于啤酒中厌氧菌检测Mannitol Salt Agar Medium
用于气单胞菌分离培养1000mlMannitol-Egg-Yolk-Polymycin Agar Base,Modified
用于气单胞菌分离培养1mlMannitol-Egg-Yolk-Polymyxin Agar Base
用于青霉,曲霉的产毒培养2mlMannitol-Egg-Yolk-Polymyxin Agar Base
用于青霉,曲霉鉴定及保存菌种用(GB标准)MannitolSalt Agar Medium
用于清汁、浊汁、水和浓缩果汁中的耐热耐酸菌的检测。125μg/支*5Martin Agar Medium, Modified
用于清汁、浊汁、水和浓缩果汁中的耐热耐酸菌的检测。250gMartin Agar Medium,Modified
用于曲霉菌分离培养Martin Broth,Modified
用于去甲万古霉素等的效价测定7ml/瓶Martin Medium, Modified
用于热死环丝菌的分离培养。2ml*20M-Broth
用于热死环丝菌的分离培养。MC Medium
用于溶血性链球菌、蜡样芽孢杆菌和金黄色葡萄球菌的增菌培养(GB标准)250gMCP Agar
Ziyuglycoside II远志皂苷B
β-d-Glucopyranoside, 5b-[(benzoyloxy)methyl]tetrahydro-5-hydroxy-2-methyl-2,5-methano-1H-3,4-dioxacyclobuta[cd]pentalen-1a(2H)-yl, [1aR-乳腺导管上皮细胞上海直销(1aα,2β,3aα,5α,5aα,5bα)]-远志皂苷元
β-D-Glucopyranoside,(3β,6α,16β,20R,24S)-3-[(3,4-di-O-acetyl-β-D-xylopyranosyl)oxy]-20,24-epoxy-16,25-dihydroxy-9,19-cyclolanostan-6-yl芸香柚皮苷
β-d-Glucopyranosiduronic acid, [3β,16α,21β(Z),22α]-22-(acetyloxy)-16,24,28-trihydroxy-21-[(2-methyl-1-oxo-2-butenyl)oxy]olean-12-en-3-yl O-β-d-glucopyranosyl-(1→2)-O-[β-d-glucopyranosyl-(1→4)]-孕甾烯醇酮;妊烯醇酮
β-Eudesmol泽泻醇A
(-)-ASARININ 97HNO3-(-)-丁香树脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷0
(-)-Epicatechin gallateC9H9NaO5(-)戈米辛L163.01284
(-)-epigallocatechinC9H8O4(+)-儿茶素117.15
(-)-Epigallocatechin gallateC9H8O3(+)松脂素-β-D-吡喃葡萄糖苷118.09
(-)-Gallocatechin gallateC9H8O2(3β,6α,16β,20R,24S)-3-O-[(3,4-二乙酰基-β-D-木糖)]-20, 24-环氧-16,25-二羟基-9,19-环羊毛甾烷-6-O-葡萄糖苷122.13
(-)-Huperzine AC9H8O(R型)人参皂苷Rg2126.11
(-)-MAACKIAINC9H6O4(R型)人参皂苷Rg3129.18
(-)scopolamine N-butyl bromideC9H6O4(R型)人参皂苷Rh1132.16
(-)-Syringaresnol-4-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranosideC9H6O4(R型)人参皂苷Rh2134.18
(+)-BicucullineC9H6O3(R型)原人参二醇136.19
(+)-Pinoresinol-4-O-beta-D-glucopyranosideC9H6O2(R型)原人参三醇137.14
(1S,2S,4R,9β,16α,17ξ)-1,2,16,20,25-pentahydroxy-9,10,14-trimethyl-4,9-cyclo-9,10-secocholest-5-ene-11,22-dioneC9H13NO2(S型)人参皂苷Rh1138.12
(24R)-3β-[6-O-(β-D-Glucopyranosyl)-β-D-glucopyranosyloxy]-24-[2-O,6-O-bis(β-D-glucopyranosyl)-β-D-glucopyranosyloxy]25-hydroxycucurbita-5-ene-11-oneC9H11NO4(S型)人参皂苷Rh2138.16