1)贴壁细胞传代:提前将培养基、PBS放入37℃水浴锅内预热,用75%酒精擦拭后再放入超净台内,吸除或倒掉细胞瓶内旧培养液,加少量PBS润洗细胞,加入适量胰酶,使胰酶的量能盖住细胞,37℃孵育,每隔2~3min显微镜下观察,待贴壁细胞间间隙变大、细胞趋于圆形但还未漂起时弃去胰酶,加入新鲜培养基,晃动细胞瓶,终止胰酶作用,用吸管小心吹打贴壁的细胞,制成细胞悬液。控制吹打的力度,避免产生大量的气泡,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养,隔天观察贴壁生长情况。
2)悬浮细胞传代:将细胞悬液转移到无菌离心管内,1000rpm离心5min,弃去上清,加入新鲜的培养基,用吸管小心吹散沉淀,制成细胞悬液,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养。
1. 收到细胞后,若发现干冰已挥发干净、冻存管瓶盖脱落、破损及细胞有污染,请立即与我们联系。
2. 所有动物细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护,所有废液及接触过此细胞的器皿需要灭菌后方能丢弃。
姜酮Acetyl Cimigenol-3-O-α-L-arabinopyranside39011-91-1662.86C24H32O6
绞股蓝皂苷3-Hydroxy-4-methoxycinnamic acid39011-92-2194.19C24H32O7
绞股蓝皂苷Aa-L-Arabinopyranoside, (3b,15a,16a,23R,24S)-16,23:16,24-diepoxy-15,25-dihydroxy-9,19-cyclolanostan-3-yl39012-04-9C24H34O4
小肠粘膜上皮细胞上海直销绞股蓝皂苷XLIXIsoacteoside39012-20-9624.58714C24H36O5
介芬胺b-D-Glucopyranoside,2-(3,4-dihydroxyphenyl)ethyl O-6-deoxy-a-L-mannopyranosyl-(13)-O-[b-D-glucopyranosyl-(16)]-, 2-acetate 4-[(2E)-3-(3,4-dihydroxyphenyl)-2-propenoate]39089-30-0C24H39NO6
芥子碱硫氰酸盐acteoside39262-14-1624.59C24H39NO7
金合欢素;刺槐素2-Acetylacteoside standard39432-56-9C24H40O4
金雀花碱;野靛碱Sec-O-Glucosylhamaudol39524-08-8436.45232C24H40O4
金色酰胺醇酯BERBAMINE DIHYDROCHLORIDE40246-10-4323.2601C24H40O5
金石蚕苷Canadine402513-88-6339.38C24H45NO10
金丝桃苷Oroxyloside40437-72-7460.39C25H22O10
金丝桃素4046-02-0C25H22O10
京尼平4H-1-Benzopyran-4-one,7-[(6-O-b-D-glucopyranosyl-b-D-glucopyranosyl)oxy]-5,6-dihydroxy-2-phenyl-404-86-4C25H22O10
京尼平苷酸1H-Pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione,4-ethyl-4-hydroxy-9-methoxy-, (4S)-40957-83-3378.38C25H22O10
酒石酸长春瑞滨10-Hydroxycamptothecin41059-79-4364.35C25H24O12
酒石酸长春质碱7-Ethyl-10-hydroxycamptothecin4168-17-6392.4C25H24O12
桔梗皂苷D41743-73-1C25H24O12
桔红素Lathyrol41753-43-9334.4498C25H24O12
菊苣酸Irinotecan41753-55-3586.69C25H24O6
巨大戟醇4H-Cyclopenta[a]cyclopropa[f]cycloundecen-4-one,4a,8-bis(acetyloxy)-7-(benzoyloxy)-1,1a,4a,5,6,7,7a,8,9,10,11,11a-dodecahydro-1,1,3,6-tetramethyl-9-methylene-,(1aR,2E,4aR,6S,7S,7aR,8R,11aS)-rel-(+)-41849-35-8522.62922C25H28O15
具栖冬青苷;长梗冬青苷Borneol4233-96-9154.25C25H28O16
决明蒽醌;美决明子素methyl 1-(beta-D-glucopyranosyloxy)-6-hydroxy-7-methyl-1,4a,5,6,7,7a-hexahydrocyclopenta[c]pyran-4-c42553-65-1390.38234C25H28O6