英文名称:BCHE;BuCHE ;Cholinesterase,butyryl(Horse serum) ;Acylcholine acyl-hydrolase;Choline esterase, butyryl;Pseudocholinesterase
CAS号:9001-08-5/9001-8-5
分子量:~440000
级别:BR
活力:≥4units/mg
产品描述:It catalyzes the hydrolysis of a number of choline esters according to the following reaction:Acylcholine+H2O to choline+acid.It is a tetrameric glycoprotein with four subunits of equal size,each having a molecular weight of 110KDa.Butyryl cholinesterase hydrolyzes butyrylcholine four times more rapidly than acetylcholine.It is inhibited by 10-5 M physostigmine,numerous organophosphate esters,the carbamate derivates and quaternary ammonium saltsunit hydrolyzes 1umole of acetylchoine per minute at 25℃, pH7.4
性状:淡褐色粉末。胆碱酯酶有2种:AChE和丁酰胆碱酯酶(BuChE)。两者的基因结构、氨基酸序列及空间三维结构均显示了很高的同源性。二者的主要差异在于,AChE水解乙酰胆碱(ACh)和乙酰硫代胆碱(ATCh)比丙酰胆碱(PrCh)快,对丁酰胆碱(BuCh)和丁酰硫代胆碱(BuTCh)没有明显的水解作用。丁酰胆碱酯酶与乙酰胆碱酯酶的显色反应原理是完全一样的。丁酰胆碱酯酶具有灵敏度更高的优点
用途:生化研究。是一类糖蛋白,水解乙酸胆碱。是生物神经传导中的一种关键性酶,同时又是有机磷和氨基甲酸酯杀虫剂的靶标。这种酶是对付神经毒气弹的QL解du品,可以使包含有神经毒剂、杀虫剂和化学熔剂的有机磷酸酯中性化,从而不受神经毒剂的侵扰
保存:2~8℃