炭疽杆菌致死因子LF抗体_详细资料

产品信息 炭疽杆菌致死因子LF抗体，应用于IHC、WB、 IF、IP、ELISA等实验，公司生产的抗体每个流程都执行严格的检测标准，保证蛋白抗原产品质量，质量稳定，实验效果明显。按理化性质和生物学功能，可将其分为IgM、IgG、IgA、IgE、IgD五类。公司产品经无数次市场验证，若出 产品编号Rs-12564R 英文名称Bacillus anthracis lethal factor 中文名称炭疽杆菌致死因子LF抗体 别名Anthrax lethal factor; Anthrax lethal toxin endopeptidase component; Anthrax LF; bacillus anthracis lethal factor; Lef; LF; LEF_BACAN. 说明书0.2ml 研究领域免疫学细菌及病毒 抗体来源Rabbit 克隆类型Polyclonal 交叉反应Anthrax LF (Lethal Factor) produced by Bacillus anthracis 产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分子量90kDa 细胞定位分泌型蛋白 性状Lyophilized or Liquid 浓度1mg/1ml 免疫原KLH conjugated synthetic peptide derived from Bacillus anthracis lethal factor 亚型IgG 纯化方法affinity purified by Protein A 储存液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol. 保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. PubMedPubMed 产品介绍background: The protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species ａnd that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates. Function: One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species ａnd that may cause death. LF is the lethal factor that, when associated with PA, causes death. LF is not toxic by itself. It is a protease that cleaves the N-terminal of most dual specificity mitogen-activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N-terminal proline-rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein-protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LeTx intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates. Subunit: Anthrax toxins are composed of three distinct proteins, a protective antigen (PA), a lethal factor (LF) ａnd an edema factor (EF). None of these is toxic by itself. PA+LF forms the lethal toxin (LeTx); PA+EF forms the edema toxin (EdTx). Subcellular Location: secreted Similarity: Belongs to the peptidase M34 family. Database links: UniProtKB/Swiss-Prot: P15917.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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炭疽杆菌致死因子LF抗体

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