鼻咽癌相关转录调节蛋白抗体，应用于IHC、WB、 IF、IP、ELISA等实验，公司生产的抗体每个流程都执行严格的检测标准，保证蛋白抗原产品质量，质量稳定，实验效果明显。按理化性质和生物学功能，可将其分为IgM、IgG、IgA、IgE、IgD五类。公司产品经无数次市场验证，若出
产品编号Rs-7779R
英文名称BRD7
中文名称鼻咽癌相关转录调节蛋白抗体
别 名75 kDa bromodomain protein; BP75; BRD 7; BRD7; BRD7_HUMAN; Bromodomain containing 7; bromodomain containing protein 7; Bromodomain-containing protein 7; CELTIX 1; CELTIX1; NAG4; Protein CELTIX-1.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 信号转导 细胞周期蛋白 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量74kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human BRD7
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, ａnd as a protein which interacts with p53 ａnd is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 ａnd 14. Alternative splicing results in multiple transcript variants.
Function:
Acts both as coactivator ａnd as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at ＇Tyr-216＇. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at ＇Lys-9＇ (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 ａnd POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at ＇Lys-382＇, ａnd thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
Subunit:
Interacts with TRIM24, PTPN13 ａnd DVL1. Identified in a complex with SMARCA4/BRG1, SMARCC1/BAF155, SMARCE1/BAF57, DPF2/BAF45D ａnd ARID2, subunits of the SWI/SNF-B (PBAF) chromatin remodeling complex (By similarity). Interacts with IRF2 ａnd HNRPUL1. Interacts (via N-terminus) with TP53. Interacts (via C-terminus) with EP300. Interacts with BRCA1. Interacts (via bromo domain) with histone H3 (via N-terminus) acetylated at ＇Lys-14＇ (H3K14ac). Has low affinity for histone H3 acetylated at ＇Lys-9＇ (H3K9ac). Has the highest affinity for histone H3 that is acetylated both at ＇Lys-9＇ (H3K9ac) ａnd at ＇Lys-14＇ (H3K14ac). Has very low affinity for non-acetylated histone H3. Interacts (via bromo domain) with histone H4 (via N-terminus) acetylated at ＇Lys-8＇ (H3K8ac) (in vitro).
Subcellular Location:
Isoform 2: Nucleus.
Similarity:
Contains 1 bromo domain.
Database links:
UniProtKB/Swiss-Prot: Q9NPI1.1
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
BRD7可能参与了基因转录调控,是一个核转录调节蛋白。经研究发现BRD7可能是通过Ras/MEK/ERK和Rb/E2F两条通路影响细胞周期的进程，使细胞停滞于G0/G1期，BRD7具有阻止细胞周期进程的作用。BRD7蛋白尤其是在鼻咽癌的发病过程中发挥了十分重要作用，BRD7基因的过表达可通过调节MAPK及Wnt信号通路YZ鼻咽癌的生长，并将鼻咽癌阻滞在G1/S期。
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