本品是由Cobuddy Super Fidelity DNA Polymerase、PCRBuffer、Mg2+、dNTPs以及PCR稳定剂和增强剂组成的预混体系,浓度为2×。Cobuddy Super Fidelity DNA Polymerase是一种快速、高扩增效率的高保真DNA聚合酶,具有5′-3′DNA聚合酶活性和3′-5′外切核酸酶活性。经过改造的聚合酶其活性区域融合有一段聚合增强结构域,独特的结构极大提升了保真性和延伸速度。Cobuddy Super Fidelity DNA Polymerase的保真性是普通Taq DNA Polymerase的50倍,是普通Pfu的6倍。其延伸速率为15-30s/kb,成功克服了普通Pfu酶扩增效率低、扩增速度慢的缺陷,大大缩短了反应时间。
本品特别适合于在基因克隆实验中扩增长片段DNA,尤其在扩增>10 kb的DNA段时,优势更加明显,所扩增的片段Z长可达20 kb。预配好的PCR混合液使操作更加简单快捷,可限度地减少人为误差和污染。
使用本产品扩增得到的PCR产物的3′端不带有“A”碱基,可直接克隆于平末端载体中。如需进行T/A克隆,需在PCR产物末端添加“A”后进行克隆。本产品具有延伸速度快、扩增效率高、保真性强的特点。适用于基因克隆、基因定点突变、SNP等实验。本产品已加入染料(蓝色),反应结束后可直接进行电泳检测,无需再使用上样缓冲液。
Cobuddy MasterMix(含染料) Cobuddy MasterMix(With Dye)
DM0680"Taq DNA Polymerase (5U/μl) (10×PCR Buffer with Mg2+)"500U
DM0681"Taq DNA Polymerase (5U/μl) (10×PCR Buffer with Mg2+)"6×500U
DM0682"Taq DNA Polymerase (5U/μl) (10×PCR Buffer with Mg2+)"5×2000U
DM0683"Taq DNA Polymerase(PAGE胶专用)Taq DNA Polymerase(For PAGE)"500U
DM0684"2×Taq MasterMix(含染料)2×Taq MasterMix(With Dye)"1ml
DM0685"2×Taq MasterMix(含染料)2×Taq MasterMix(With Dye)"5×1ml
DM0686"2×Taq MasterMix(含染料)2×Taq MasterMix(With Dye)"10×5ml
DM0687"2×Taq MasterMix(无染料)2×Taq MasterMix(Without Dye)"1ml
DM0688"2×Taq MasterMix(无染料)2×Taq MasterMix(Without Dye)"5×1ml
DM0689"2×Taq MasterMix(无染料,PAGE胶专用)2×Taq MasterMix(Without Dye,For PAGE)" 1ml
DM0690"2×Taq MasterMix(无染料,PAGE胶专用)2×Taq MasterMix(Without Dye,For PAGE)" 5×1ml
DM0691"Es Taq DNA Polymerase (5U/μl) (10×Es Taq Buffer with Mg2+)"500U
DM0692"Es Taq DNA Polymerase (5U/μl) (10×Es Taq Buffer with Mg2+)"6×500U
DM0693"Es Taq DNA Polymerase (5U/μl) (10×Es Taq Buffer with Mg2+)"5×2000U
DM0694"2×Es Taq MasterMix(含染料)2×Es Taq MasterMix(With Dye)"1ml
DM0695"2×Es Taq MasterMix(含染料)2×Es Taq MasterMix(With Dye)"5×1ml
DM0696"2×Es Taq MasterMix(含染料)2×Es Taq MasterMix(With Dye)"10×5ml
DM0697"2×Es Taq MasterMix(无染料)2×Es Taq MasterMix(With Dye)"1ml
DM0698"2×Es Taq MasterMix(无染料)2×Es Taq MasterMix(With Dye)"5×1ml
DM0699"2×Es Taq MasterMix(无染料,PAGE胶专用)2×Es Taq MasterMix(Without Dye, For PAGE)"1ml
DM0700"2×Es Taq MasterMix(无染料,PAGE胶专用)2×Es Taq MasterMix(Without Dye, For PAGE)"5×1ml