Anti-GDNF Receptor alpha 2胶质细胞系源性神经营养因子受体α2抗体

本公司经销GDNF Receptor alpha 2，胶质细胞系源性神经营养因子受体α2抗体，克隆类型为polyclonal，宿主来源是Rabbit，GDNF Receptor alpha 2胶质细胞系源性神经营养因子受体α2抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0054R
英文名称：Anti-GDNF Receptor alpha 2
中文名称：胶质细胞系源性神经营养因子受体α2抗体
其他名称：名GDNF family receptor alpha 2; GDNF Receptor α2;GDNF receptor beta; GDNFR beta; GDNFRB; GFR alpha 2; GFRA 2; GFRA2; Glial cell line derived neurotrophic factor family receptor alpha2b; Glial cell line derived neurotrophic factor receptor beta; Neurturin receptor alpha; NRTNR alpha; NTNR alpha; NTNRA; PI linked cell surface accessory protein; RET ligand 2; RETL 2; RETL2; TGF beta related neurotrophic factor receptor 2; TRN receptor GPI anchored; TRNR 2; TRNR2.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 49kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, cow, dog, hrs, chk
产品介绍：Glial cell line-derived neurotrophic factor （GDNF） ａnd neurturin （NTN） are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival ａnd differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol（GPI）-linked cell surface receptor for both GDNF ａnd NTN, ａnd mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene.Members of the glial cell line-derived neurotrophic factor（GDNF） family, including GDNF ａnd neurturin （NTN）,play key roles in the control of vertebrate neuron survivaland differentiation. Physiological responses to NTN requirethe presence of a novel glycosylphosphadidylinositollinked protein NTNRa, which is a cell surface receptor forNTN. The cDNAs encoding NTNRa from human, rat,chicken, ａnd mouse have been cloned recently. NTNRa was also termed GDNFRb, Ret ligand 2 （RETL2）or TGF-b-related neurotrophic factor receptor 2 （TrnR2） ａnd nominated as GFRa-2 recently. GFRa-2 binds NTN ａnd mediates activation of RET receptor tyrosine kinase by both NTN ａnd GDNF. Thus, NTN, GFRa-2, ａnd the Ret PTK form a complex to transduce NTN signal andto mediate NTN function.Function : Receptor for neurturin. Mediates the NRTN-induced autophosphorylation ａnd activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.Subcellular Location : Cell membrane; Lipid-anchor, GPI-anchor （By similarity）.Tissue Specificity : Isoform 1 is found in both brain ａnd placenta.Similarity : Belongs to the GDNFR family.神经生物学相关蛋白（Neurobiology） 胶质细胞源性神经营养因子（GDNF）是TGF-β家族的一个新亚族，该亚族还有新发现的三个成员：Neurturin（NTN）、Persephin（PSP）和Artemin（ART），它们均不程度地对多巴胺神经元、脊髓前角运动神经元、背根神经节、颈上神经节以及肾等非神经元细胞都具有生长促进作用。研究表明胶质细胞系源性神经营养因子,主要用于PD的研究。GDNF的受体是多成分的复合物，它是由固定于质膜外层的糖基磷脂酰肌醇（GPI）被称为GDNFRα和酪氨酸激酶RET蛋白组成。GDNFRα为其配体结合亚单位，而RET则是GDNF的功能性受体亚单位，即GDNF特异地结合于其受体的GDNFRα上，再激活RET的酪氨酸磷酸化传导其信号，影响细胞的活性，发挥其生物学效应。目前，又克隆出了人与大鼠的GDNFRβ序列，与GDNPRα具有47％的同源性。GDNFRβ mRNA在脊髓、DRG和发育中的周围神经上也均有表达。产品图片Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-GDNF-Receptor-alpha-2 Polyclonal Antibody, Unconjugated（bs-0054R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-GDNF-Receptor-alpha-2 Polyclonal Antibody, Unconjugated（bs-0054R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingTissue/cell: human placenta tissue;4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-GDNF-Receptor-alpha-2 Polyclonal Antibody, Unconjugated（bs-0054R） 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated （bs-0295G-PE）used at 1:200 dilution for 40 minutes at 37°C. Antigen: bs-0054P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG（bs-0295G-HRP） at 1: 5000; TMB（C-0024） staining; Read the data in MicroplateReader by 450nm.