Anti-NFBD1/MDC1DNA损伤关卡蛋白1抗体

本公司经销NFBD1/MDC1，DNA损伤关卡蛋白1抗体，克隆类型为polyclonal，宿主来源是Rabbit，NFBD1/MDC1DNA损伤关卡蛋白1抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-2400R
英文名称：Anti-NFBD1/MDC1
中文名称：DNA损伤关卡蛋白1抗体
其他名称：名Nuclear factor with BRCT domains protein 1; DKFZp781A0122; Homologue to Drosophila photoreceptor protein calphotin; KIAA0170; MGC166888; MDC 1; Mediation of DNA damage checkpoint 1; Mediator of DNA damage checkpoint 1; Mediator of DNA Damage Checkpoint Protein 1; NFBD 1; NFBD1; Nuclear Factor with BRCT Domains Protein 1.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 226（human）, 188（mo）, 147（rat）kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, hrs, cow, pig, dog （MBS）
产品介绍：The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal （BRCT） motifs ａnd a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase ａnd G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, ａnd facilitates recruitment of the ATM kinase ａnd meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq].Function : Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase ａnd G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair ａnd signal transduction proteins to discrete foci of DNA damage marked by ＇Ser-139＇ phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation ａnd activation of the ATM, CHEK1 ａnd CHEK2 kinases, ａnd stabilization of TP53 ａnd apoptosis. ATM ａnd CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.Subunit : Interacts with several proteins involved in the DNA damage response, although not all these interactions may be direct. Interacts with H2AFX, which requires phosphorylation of H2AFX on ＇Ser-139＇. Interacts with the MRN complex, composed of MRE11A/MRE11, RAD50, ａnd NBN. Interacts with CHEK2, which requires ATM-mediated phosphorylation of ＇Thr-68＇ within the FHA domain of CHEK2. Interacts constitutively with the BRCA1-BARD1 complex, SMC1A ａnd TP53BP1. Interacts with ATM ａnd FANCD2, ａnd these interactions are reduced upon DNA damage. Also interacts with the PRKDC complex, composed of XRCC6/KU70, XRCC5/KU80 ａnd PRKDC/XRCC7. This interaction may be required for PRKDC autophosphorylation, which is essential for DNA double strand break （DSB） repair. When phosphorylated by ATM, interacts with RNF8 （via FHA domain）. Interacts with CEP164. When phosphorylated, interacts with APTX （via FHA-like domain）.Subcellular Location : Nucleus. Note=Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires ＇Ser-139＇ phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.Tissue Specificity : Highly expressed in testis.Post-translational modifications : Phosphorylated upon exposure to ionizing radiation （IR）, ultraviolet radiation （UV）, ａnd hydroxyurea （HU）. Phosphorylation in response to IR requires ATM, NBN, ａnd possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle ａnd during activation of the mitotic spindle checkpoint.Similarity : Contains 2 BRCT domains.Contains 1 FHA domain.NFBD1又称MDC1（mediator of DNA damagecheckpoint protein 1）是一个参与DNA损伤细胞应答通路的重要传递和修复因子,在DNA损伤关卡、DNA损伤修复、细胞生长增殖及肿瘤发生中均有作用。