NF-H/Neurofilament H/Neurofilament 200高分子量神经丝蛋白抗体

本公司经销NF-H/Neurofilament H/Neurofilament 200，高分子量神经丝蛋白抗体，克隆类型为polyclonal，宿主来源是Rabbit，NF-H/Neurofilament H/Neurofilament 200高分子量神经丝蛋白抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0708R
英文名称：Anti-NF-H/Neurofilament H/Neurofilament 200
中文名称：高分子量神经丝蛋白抗体
其他名称：名200 kDa neurofilament protein; 200 kD Neurofilament Heavy; NEFH; NEFH; NF200; Neurofilament H; Neurofilament heavy polypeptide 200kD; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; Neurofilament triplet H protein; NF H; NF-H; NFH; NFH_HUMAN; KIAA0845.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 118kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat
产品介绍：Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons ａnd their synapses ａnd to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light （NFL）, neurofilament medium （NFM） ａnd neurofilament heavy （NFH）. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin ａnd in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, ａnd are biochemically very stable. Some studies have shown that levels of neurofilament heavy ａnd neurofilament light are elevated in patients with Alzheimer＇s disease, frontotemporal lobe dementia, ａnd vascular dementia.Function : Neurofilaments usually contain three intermediate filament proteins: L, M, ａnd H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.Post-translational modifications : There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides （NF-M ａnd NF-H）, the levels of phosphorylation being altered developmentally ａnd coincidentally with a change in the neurofilament function.Phosphorylated in the head ａnd rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.DISEASE : Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis （ALS） [MIM:105400]. ALS is a neurodegenerative disorder affecting upper ａnd lower motor neurons, ａnd resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic ａnd environmental factors.Similarity : Belongs to the intermediate filament family.神经生物学相关蛋白（Neurobiology） 高分子量神经丝蛋白,简称NF-H,分子量为200kDa 神经丝是中间纤维的一种重要类型又称神经微丝蛋白，特异地在神经细胞内表达，并在轴突内相互平行排列成束.　哺乳动物的神经丝由3种蛋白组成： 低分子量神经丝蛋白，简称NF-L；分子量为68kDa； 中分子量神经丝蛋白，简称NF-M；分子量为160kDa； 高分子量神经丝蛋白，简称NF-H，分子量为200kDa； 经纤丝蛋白的功能是提供弹性使神经纤维易于伸展和防止断裂。NF是神经元特异性中间丝蛋白，由三个不同分子量的亚单位构成的多聚体，以不同的比例分布于、外周神经元以及肿瘤中，其阳性主要于节细胞神经瘤、副节瘤、小脑或外周神经母细胞瘤、肾上腺瘤或外周嗜铬细胞瘤，有助于神经母细胞瘤和嗜铬细胞瘤的诊断与鉴别诊断。 产品图片Tissue/cell: rat spinal cord tissue;4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-NF-H/Neurofilament H/Neurofilament 200 Polyclonal Antibody, Unconjugated （bs-0708R） 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated（bs-0295G-FITC）used at 1:200 dilution for 40 minutes at 37°C. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-NF-H/Neurofilament H/Neurofilament 200 Polyclonal Antibody, Unconjugated （bs-0708R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） staining