博研生物试剂提供售前、售中、售后免费技术服务,欢迎致电索取说明书等相关产品资料
中文名称: SuperScriptⅡ阴性逆转录酶,促销价格
英文名称:SuperScript II Reverse Transcriptase
级别:BR
来源:Purified from E. coli expressing the pol gene of
M-MLV (1,2), mutagenized to reduce the RNase H activity
浓度:200u/ul
活力定义:One unit of SuperScript® II is the amount of enzyme
required to incorporate 1 nmole of eoxyribonucleotide
into acid-precipitable material in 10 min. at 37°C using
poly(A)•oligo(dT)12-18 as template•primer (3).
Unit Reaction Conditions:50 mM Tris-HC1 (pH 8.3), 40 mM
KC1, 6 mM MgC1 2 , 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM
poly(A), 0.1 mM oligo(dT)12-18 , 0.1 mg/m1 BSA, and
enzyme in 50 µl for 10 min. at 37℃
Performance and Quality Testing:SDS-PAGE
purity;endodeoxyribonuclease, exodeoxyribonuclease, and
ribonuclease assays; and yield and length of cDNA
product
Enzyme Function:RNA dependent DNA Polymerase
Final Product Size:12.3 kb or less
Number of Reactions:10 Reactions
Optimal Reaction Temperature:42℃
Sensitivity:Medium
Template:ssRNA;ssDNA;RNA-DNA Hybrid
产品描述:SuperScript® II Reverse Transcriptase (RT) is an
improved version of SuperScript® RT. It is a DNA
polymerase that synthesizes a complementary DNA strand
from single-stranded RNA, DNA, or an RNA:DNA hybrid.
This enzyme is genetically engineered by the
introduction of point mutations rather than a deletion
in the RNase H active center. Like SuperScript® RT,
SuperScript® II RT has reduced RNase H activity. Unlike
SuperScript® RT, however, the selective mutations within
the RNase H domain maintain full polymerase activity.
This structural modification eliminates degradation of
RNA molecules during first-strand cDNA synthesis and
gives SuperScript® II RT superior performance
characteristics, including:Greater first-strand cDNA
yields;More full-length cDNA synthesis;Full activity at
42℃
性状:悬浮液
用途:生化研究。
保存:-20℃
客服电话:021 60340850 021 60340830 021 60340820
客服QQ :827774711 349199432 372584443
SuperScriptⅡ阴性逆转录酶,促销价格试剂的取用规则
固体粉末试剂可用洁净的牛角勺取用。要取一定量的固体时,可把固体放在纸上或表面皿上在台秤上称量。要准确称量时,则用称量瓶在天平上进行称量。液体试剂常用量筒量取,量筒的容量为:5mL、10mL、50mL、500mL等数种,使用时要把量取的液体注入量筒中,使视线与量筒内液体凹面的处保持水平,然后读出量筒上的刻度,即得液体的体积。 如需少量液体试剂则可用滴管取用,取用时应注意不要将滴管碰到或插入接收容器的壁上或里面。
为了达到准确的实验结果,取用试剂时应遵守以下规则,以保证试剂不受污染和不变质:
(1)试剂不能与手接触。
(2)要用洁净的药勺,量筒或滴管取用试剂,不准用同一种工具同时连续取用多种试剂。取完一种试剂后,应将工具洗净(药勺要擦干)后,方可取用另一种试剂。
(3)试剂取用后一定要将瓶塞盖紧,不可放错瓶盖和滴管,绝不允许张冠李戴,用完后将瓶放回原处。
(4)已取出的试剂不能再放回原试剂瓶内。
另外取用试剂时应本着节约精神,尽可能少用,这样既便于操作和仔细观察现象,又能得到较好的实验结果
SuperScriptⅡ阴性逆转录酶,促销价格低价优惠促销活动, 查询或联系在线客服人员联系方式点击此处>>>>
生物试剂(Biochemical reagent)是指有关生命科学研究的生物材料或有机化合物,以及临床诊断、医学研究用的试剂。由于生命科学面广、发展快,因此该类试剂品种繁多、性质复杂。主要有电泳试剂、色谱试剂、离心分离试剂、免疫试剂、标记试剂、组织化学试剂、透变剂和致癌物质、杀虫剂、培养剂、缓冲剂、电镜试剂、蛋白质和核酸沉淀剂、缩合剂、超滤膜、临床诊断试剂、染色剂、抗氧化剂、防霉剂、去垢剂和表面活性剂、生化标准品试剂、生化质控品试剂、分离材料等等。