StemRD  PSGro 人iPS细胞和胚胎干细胞无血清培养基

StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基 500mL/套

产品英文名称：StemRD PGroF-500 PSGro-case, Human iPSC/ESC Growth Medium

产品中文名称：StemRD 人iPS细胞和胚胎干细胞无血清培养基,无异源级别

StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基产品特点：

★ Serum-Free 无血清成分；

★ Xeno-Free 无异种蛋白；

★ Chemically-Defined 化学成分明确；

★ Feeder-Independent 无需滋养层细胞；

★ Supports human iPSCs/ESCs 用于人iPS细胞和胚胎干细胞生长；

★ Performs Comparably to mTeSR 1 效能与mTeSR 1培养基同等。

Characteristic Morphology

Human ESCs(A,B); iPSCs(C,D) in PSGro-Free®

PSGro-Free 是stemRD开发的一款针对人类胚胎干细胞

（ESCs）和多能诱导干细胞（iPSCs）的生长维护培养

基。我们生产质量可靠的生长因子，并拥有来自System

Biosciences的iPS研究专家。PSGro-Free的所有添

加组分都为化学合成或已纯化的重组蛋白，并且决不含有

任何非人源的异种成分。

PSGro-Free is a growth medium designed for human

induced pluripotent stem cells ( iPSCs ) ａnd human

embryonic stem cells (ESCs). It was developed at

StemRD, a premier producer of growth factors, in

collaboration with iPS experts at System Biosciences.

红荣微再（上海）生物工程技术有限公司主营：干细胞、JZYL、细胞ZL、器官再生 四大板块的产品，以“传递科学价值，服务科学研究”为宗旨，经过近两年的努力推广，红荣微再（上海）生物工程技术有限公司在全ZG代理的CellArtis干细胞产品已经成功销往：上海市、北京市、重庆市、天津市、广东省的广州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、绵阳、浙江省的杭州市、宁波、温州、江苏省的南京市、苏州、无锡、泰州、湖南省的长沙、陕西省的西安市、杨凌、甘肃省的兰州、宁夏的银川、新疆的乌鲁木齐、广西省的南宁市、山东省的济南、青岛、东北三省 辽宁省的沈阳市、大连市、吉林省的长春市、黑龙江省的哈尔滨市等ZG绝大部分省市和自治区。

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  手机号：152 1681 4001。华雅再生医学-客服QQ: 316 808 6348

StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基 产品英文描述如下：

PSGro-case is designed for human induced pluripotent stem cells (iPSCs) ａnd human embryonic stem cells (ESCs). It is serum-free ａnd chemically-defined as all of its ingredients are synthetic chemicals or purified recombinant proteins. PSGro-case supports feeder-free growth ａnd maintenance of human iPSCs or human ESCs on extra-cellular matrix proteins (e.g., BD Matrigel?). Its performance is comparable to or better than the market-leading mTeSR?1 or E8 in growth rate, characteristic morphology, pluoripotent markers, normal karyotype, ａnd differentiation to different germ layers. The robustness ａnd stability of PSGro-case enable seeding cells over the weekend without changing medium, so you have the weekend Free.

StemRD PGroF-500 PSGro-case, Human iPSC/ESC Growth Medium 产品组分：

提供产品：华雅再生医学旗舰公司-红荣微再（上海）生物工程技术有限公司为ZG再生医学和生物工程技术领域的组织器官再生研究、组织器官功能重建研究、干细胞技术研究、组织工程生物支架材料研究、器官移植YL器械产品等再生医学产业链的相关客户提供：iPS/ES胚胎干细胞的种子细胞、心肌细胞、人源肝脏细胞、MSC间充质干细胞、基底膜基质胶细胞支架、干细胞生长分化调控因子、干细胞药物研究的小分子YZ剂、调节剂、小分子化合物、功能胶原蛋白生物材料、3D细胞培养基质胶、器官模型3D打印技术材料、神经干细胞培养基、STEM121抗体、BD科研P100、P800蛋白质组学采血管、BD血浆准备管PPT管、BD PAXgene全血RNA样本收集管、BD公司CPT/PRP美容采血管、Streck Cell-Free RNA BCT游离RNA样本收集管、Streck Cell-Free DNA Urine Preserve游离DNA尿液保存管、Streck Cell-Free DNA BCT游离DNA采血管、循环肿瘤细胞CTC及无创产前基因检测采血管、illumina高品质NGS文库制备试剂盒、ThruPLEX ctDNA测序试剂盒、全基因组测序、全外显子测序、靶向测序、ChIP-Seq测序、CNV和染色体异倍性分析、Rubicon Genomics单细胞全基因组测序试剂盒、生物医学试剂耗材仪器等种类丰富的产品，以“Focus on Tissue ａnd Organ Regeneration专注组织器官再生与功能重建”为己任，我们与客户携手为ZG再生医学产业的发展而前行。

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  手机号：152 1681 4001。华雅再生医学-客服QQ: 316 808 6348

Content ａnd Storage: Components  Size  Storage   Shelf Life

Basal Medium (on gel ice)   450mL    2 to 8°C. Keep from light   6 months

10x Supplement (dry ice)  50mL    -20°C or below. Keep from light  6 months      

Additional key reagents required or suggested

Extracellular matrix proteins, such as Matrigel  (BD, cat# 354277) or equivalent

Accutase (Millipore, SCR005) or Collagenase IV (Invitrogen, 17104) or equivalent

ROCK inhibitor Y-27632 (StemRD, Y-01/-05/-25) or Thiazovivin (StemRD, Thia-01/-05/-25)

Medium Preparation

1. Thaw PSGro-case Supplement overnight at 4°C. If desired, thawed Supplement can be aliquoted ａnd stored at -20°C or below, but further freeze-thaw should be avoided.

2. To make PSGro-case complete medium, add 1 part of Supplement to 9 parts of Basal Medium.

3. Antibiotics can be added to the complete medium if so desired.

4. Once made, PSGro-case  complete medium is stable for 2 weeks when stored in dark at 2 to 8°C.

StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基 评价或评论

红荣微再（上海）生物工程技术有限公司在ZG代理销售的StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基是从美国进口的，由美国StemRD公司研发生产。美国StemRD公司是在经美国FDA认证的GMP实验室里面采用人类细胞和原核表达系统进行重组蛋白的表达生产与纯化工作，同时该GMP实验室拥有美国生物技术领域高度的仪器设备，确保产品质量的稳定可靠，纯度和活性优于同行，StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基的价格合理，与加拿大StemCell Technologies Inc,公司的mTeSR 1 Feeder-Independent Growth of hESCs ａnd hiPSCs人胚胎干细胞和iPS细胞无血清培养基相比较，可谓性价比更高。红荣微再（上海）生物工程技术有限公司在上海仓库备有少量StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基现货，并严格按照产品的储存条件进行储存与管理，我们的一贯宗旨是：更用心地为ZG广大科学研究工作者提供更安全、更放心、更便捷的服务。

Coating plates with extracellular matrix proteins, such as Matrigel?

Refer to manufacturers’ instructions. For BD Matrigel?, the following procedure can be followed:

1. Thaw Matrigel? on ice. Dilute Matrigel 1:50 with pre‐chilled DMEM medium.

2. Immediately use the diluted Matrigel? solution to coat tissue culture plates. For a 6‐well plate, use 1 mL of diluted Matrigel? solution per well, ａnd swirl the plate to spread the solution evenly.

3. Let the plate stand for 1 h at 37°C or overnight at 4°C.

Adaptation of growing feeder-free cells to PSGro-case

Human iPSC or ESCs that have been grown in most commonly-used hiPSC/ESC media can be easily adapted into PSGro-case. One round of 1:1 dilution of serum-containing or serum-free medium with PSGro-case is generally sufficient. However, for certain media ａnd cell lines, a longer ａnd more graduate adaptation may be required.

Adaptation of growing feeder-dependent cells to PSGro-case

1. Dissociate cells with Accutase, Collagenase IV or equivalent.

2. Add the previously-used medium ａnd gently scrape off cells as aggregates. The ideal aggregate size is similar to other media ａnd single cells from excessive pipetting may lower plating efficiency.

3. Transfer the suspension with cell aggregates into a 15 ml tube.

4. Centrifuge at 200 x g for 5 minutes at room temperature.

5. Discard Matrigel? solution, immediately add PSGro-case (e.g., 2 mL/well for a 6-well plate).

6. After centrifugation, discard the supernatant, gently resuspend the pellet with 2 mL of the previously-used medium. Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival ａnd plating efficiency.

7. Transfer the cell aggregates in the previously-used medium to the wells with PSGro-case. Mix. Gently.

8. Culture the cells at 37°C, with 5% CO2 ａnd 95% humidity.

9. If cells are seeded on Friday, change medium on Monday to 4 mL PSGro-case (no need to change over the weekend). If cells are seeded on other days during the week, change next day.

提供产品：华雅再生医学旗舰公司-红荣微再（上海）生物工程技术有限公司为ZG再生医学和生物工程技术领域的组织器官再生研究、组织器官功能重建研究、干细胞技术研究、组织工程生物支架材料研究、器官移植YL器械产品等再生医学产业链的相关客户提供：iPS/ES胚胎干细胞的种子细胞、心肌细胞、人源肝脏细胞、MSC间充质干细胞、基底膜基质胶细胞支架、干细胞生长分化调控因子、干细胞药物研究的小分子YZ剂、调节剂、小分子化合物、功能胶原蛋白生物材料、3D细胞培养基质胶、器官模型3D打印技术材料、神经干细胞培养基、STEM121抗体、BD科研P100、P800蛋白质组学采血管、BD血浆准备管PPT管、BD PAXgene全血RNA样本收集管、BD公司CPT/PRP美容采血管、Streck Cell-Free RNA BCT游离RNA样本收集管、Streck Cell-Free DNA Urine Preserve游离DNA尿液保存管、Streck Cell-Free DNA BCT游离DNA采血管、循环肿瘤细胞CTC及无创产前基因检测采血管、illumina高品质NGS文库制备试剂盒、ThruPLEX ctDNA测序试剂盒、全基因组测序、全外显子测序、靶向测序、ChIP-Seq测序、CNV和染色体异倍性分析、Rubicon Genomics单细胞全基因组测序试剂盒、生物医学试剂耗材仪器等种类丰富的产品，以“Focus on Tissue ａnd Organ Regeneration专注组织器官再生与功能重建”为己任，我们与客户携手为ZG再生医学产业的发展而前行。

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  手机号：152 1681 4001。华雅再生医学-客服QQ: 316 808 6348

Recovery of frozen cells in PSGro-case

1. Quickly thaw hiPSCs or hESCs in a 37°C water bath, transfer the contents to a 15 mL tube. Add 10 mL of the medium previously-used for the cells drop-wise to the tube with gentle mixing.

2. Centrifuge cells at 200 x g for 5 minutes at room temperature.

3. Discard Matrigel solution, immediately add PSGro-case  (e.g., 2 mL/well for a 6-well plate).

4. After centrifugation, discard the supernatant, gently resuspend the pellet with 2 mL of the previously-used medium. Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival ａnd plating efficiency.

5. Transfer the cell aggregates in the previously-used medium to the plate with PSGro-case . Mix gently.

6. Culture the cells at 37°C, with 5% CO2 ａnd 95% humidity.

7. Change medium to 4 mL PSGro-case next day.

Passaging cells in PSGro-case  under feeder-free condition

1. Look under microscope to identify regions of differentiation. Mark the differentiated colonies using lens marker on the bottom of the plate (e.g., 6-well plate)

2. Remove regions of differentiation by scraping with a pipette tip or by aspiration.

3. Aspirate medium from the cell culture ａnd rinse with PBS.

4. Add 0.5 mL per well of Accutase or Collagenase IV. Let stand at room temp for 1 – 2 min.

5. Remove Accutase, ａnd gently rinse 2 – 3 times with 2 mL PSGro-case to remove remaining

enzymes.

6. Add 2 mL/well PSGro-case and scrape colonies off with a cell scraper.

7. Transfer the detached cell aggregates to a 15 mL conical tube ａnd rinse the well with an additional

2 mL PSGro-case to collect any remaining aggregates.

8. Centrifuge at 200 x g for 5 minutes at room temperature.

9. Aspirate the supernatant. Resuspend pellet in 4 mL PSGro-case by pipetting gently.

Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival ａnd plating efficiency.

10. Plate the cell aggregates with PSGro-case onto a new plate coated with Matrigel.

11. Culture the cells at 37°C, with 5% CO2 ａnd 95% humidity. Change medium daily.

12. If cells are seeded on Friday, change medium on Monday to 4 mL PSGro-case (no need to change over the weekend). If cells are seeded on other days during the week, change next day.

Note: If colonies are at an optimal density, cells can be split every 5 ‐ 7 days using 1:5 to 1:10 splits.

Cryopreservation of cells in PSGro-case

1. Prepare freezing medium which contains 90% PSGro-case and 10% DMSO.

Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival ａnd plating efficiency.

2. Perform steps 1 - 8 from Passaging cells in PSGro-case under feeder-free condition

3. Gently aspirate the supernatant taking care to keep the cell pellet intact.

4. Gently resuspend the pellet in freezing medium, taking care to leave the cell aggregates larger than would normally be done for passaging.

5. Transfer 1 mL of cell aggregates in freezing medium into each labeled cryovial.

6. Place vials into an isopropanol freezing container ａnd place the container at ‐80°C overnight.

7. Transfer to a liquid nitrogen tank the next day for long-term storage.

红荣微再（上海）生物工程技术有限公司主营：干细胞、JZYL、细胞ZL、器官再生 四大板块的产品，以“传递科学价值，服务科学研究”为宗旨，经过近两年的努力推广，红荣微再（上海）生物工程技术有限公司在全ZG代理的CellArtis干细胞产品已经成功销往：上海市、北京市、重庆市、天津市、广东省的广州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、绵阳、浙江省的杭州市、宁波、温州、江苏省的南京市、苏州、无锡、泰州、湖南省的长沙、陕西省的西安市、杨凌、甘肃省的兰州、宁夏的银川、新疆的乌鲁木齐、广西省的南宁市、山东省的济南、青岛、东北三省 辽宁省的沈阳市、大连市、吉林省的长春市、黑龙江省的哈尔滨市等ZG绝大部分省市和自治区。

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  手机号：152 1681 4001。华雅再生医学-客服QQ: 316 808 6348