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A7156 mTOR (Phospho-Ser2448) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 288 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
Product Name | mTOR (Phospho-Ser2448) Antibody |
Synonyms | FRAP; FRAP1; FRAP2; RAFT1; Rapamycin target protein; kinase mTOR |
Immunogen | The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Ser2448. |
Immunogen Region | C-terminal |
Clonality | Polyclonal |
Modification sites | H:S2448 M:S2448 R:S2448 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 |
Specificity | mTOR (Phospho-Ser2448) Antibody detects endogenous levels of mTOR only when phosphorylated at serine2448. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | MTOR |
Database Links | Gene ID: | 2475 | Swiss-Prot #: | P42345 | OMIM #: | 601231 | Unigene #: | Hs.338207 | |
Application images | Immunohistochemistry analysis of paraffin-embedded human brain, using mTOR (Phospho-Ser2448) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30', using mTOR (Phospho-Ser2448) Antibody. The lane on the right is treated with the synthesized peptide. |
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