A0054 BTK (Phospho-Tyr223) Antibody_详细资料

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A0054    BTK （Phospho-Tyr223） Antibody

Reactivity Applications MW. Source

H, M, R WB ELISA 76 kDa Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name BTK （Phospho-Tyr223） Antibody

Synonyms AGMX1; ATK; Agammaglobulinaemia tyrosine kinase; B cell progenitor kinase; BPK; Bruton＇s tyrosine kinase; Kinase EMB; kinase Btk

Immunogen The antiserum was produced against synthesized peptide derived from human BTK around the phosphorylation site of Tyr223.

Immunogen Region Internal

Clonality Polyclonal

Modification sites H:Y223 M:Y223 R:Y224

Application dilutions WB: 1:500~1:1000 ELISA: 1:40000

Specificity BTK （Phospho-Tyr223） Antibody detects endogenous levels of BTK only when phosphorylated at tyrosine223.

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

Physical Form Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

Concentration 1 mg/ml

Storage/Stability Store at -20℃/1 year

NCBI Gene Symbol BTK

Database Links

Gene ID: 695 Swiss-Prot #: Q06187

OMIM #: 300300 / 300755 / 307200 Unigene #: Hs.159494

Application images
Western blot analysis of extracts from HeLa cells treated with Serum 10% 15＇, using BTK （Phospho-Tyr223） Antibody. The lane on the right is treated with the synthesized peptide.

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Product Name	BTK （Phospho-Tyr223） Antibody
Synonyms	AGMX1; ATK; Agammaglobulinaemia tyrosine kinase; B cell progenitor kinase; BPK; Bruton＇s tyrosine kinase; Kinase EMB; kinase Btk
Immunogen	The antiserum was produced against synthesized peptide derived from human BTK around the phosphorylation site of Tyr223.
Immunogen Region	Internal
Clonality	Polyclonal
Modification sites	H:Y223 M:Y223 R:Y224
Application dilutions	WB: 1:500~1:1000 ELISA: 1:40000
Specificity	BTK （Phospho-Tyr223） Antibody detects endogenous levels of BTK only when phosphorylated at tyrosine223.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form	Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year