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A0057 C/EBP-beta (Phospho-Thr235/188) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 36 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
Product Name | C/EBP-beta (Phospho-Thr235/188) Antibody |
Synonyms | AGP/EBP; C/EBP beta; C/EBP-related protein 2; CCAAT/enhancer binding protein beta; CEBPB; CRP2; IL-6DBP; Interleukin-6- dependent binding protein; LAP; Liver-enriched transcriptional activator; Nuclear factor NF-IL6; SF-B; SFB; Silencer factor B; TCF5; Transcription factor 5 |
Immunogen | The antiserum was produced against synthesized peptide derived from human C/EBP-beta around the phosphorylation site of Thr235/188. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:T235 M:T188 R:T189 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 |
Specificity | C/EBP-beta (Phospho-Thr235/188) Antibody detects endogenous levels of C/EBP-beta only when phosphorylated at threonine235/188. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | CEBPB |
Database Links | Gene ID: | 1051 | Swiss-Prot #: | P17676 | OMIM #: | 189965 | Unigene #: | Hs.517106 / Hs.701858 / Hs.712709 | |
Application images |  Immunofluorescence analysis of HepG2 cells, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is treated with the synthesized peptide.  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30', using C/EBP-beta (Phospho-Thr235/188) Antibody. The lane on the right is treated with the synthesized peptide. |
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