CaBP29K Human/重组人钙视网膜蛋白_详细资料

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CaBP29K Human/重组人钙视网膜蛋白

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CaBP29K Human/重组人钙视网膜蛋白
产品编号：PRO-401
产品规格：1μg/5μg/100μg

Synonyms Calretinin, CR, CALB2, CAB29, CAL2, CaBP29K, 29 kDa calbindin.

Description Calretinin/CR/CLB2/CALB2/Calbindin D29K protein （mouse/rat/human 271aa, chromosome 16q22.1, mol wt ~29 kDa） also belongs to the Calbindin family. It is most closely related to CABP28K （~55% identity）. It may be alternatively spliced to a C-terminally truncated fragment, Calretinin-22K in some tumor cell lines. Calretinin is highly expressed in the cerebellum, olfactory bulb, ａnd in auditory neurons. Calretinin gene inactivation in mice eliminated long-term potentiation induction in the gentate gyrus ａnd impaired motor coordination.
Recombinant Human Calbindin 29kDa is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques.

Source Escherichia Coli.

Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder.

Formulation The protein was lyophilized from a concentrated solution （1mg/ml） containing 10mM Tris pH-7.4, 50mM sodium chloride ａnd 1mM b-mercaptoethanol.

Solubility It is recommended to reconstitute the lyophilized CABP29K in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

Stability Lyophilized CABP29K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP29K should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.

Purity Greater than 90% as determined by SDS-PAGE.

Applications This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection.
For adsorption we suggest the following procedure
A- Dilute 1 μl of the antiserum against calretinin in 5 ml of the usual buffer for immunohistochemistry （final dilution 1:5＇000）.
B- Add 1μg of recombinant protein to 1ml of the diluted antibody solution ａnd mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections ａnd incubate for 3 days.
E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）.
As a result, the immunostaining should be strongly reduced or even completely prevented.

Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Synonyms	Calretinin, CR, CALB2, CAB29, CAL2, CaBP29K, 29 kDa calbindin.
Description	Calretinin/CR/CLB2/CALB2/Calbindin D29K protein （mouse/rat/human 271aa, chromosome 16q22.1, mol wt ~29 kDa） also belongs to the Calbindin family. It is most closely related to CABP28K （~55% identity）. It may be alternatively spliced to a C-terminally truncated fragment, Calretinin-22K in some tumor cell lines. Calretinin is highly expressed in the cerebellum, olfactory bulb, ａnd in auditory neurons. Calretinin gene inactivation in mice eliminated long-term potentiation induction in the gentate gyrus ａnd impaired motor coordination. Recombinant Human Calbindin 29kDa is expressed with a -6xHis tag ａnd purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	The protein was lyophilized from a concentrated solution （1mg/ml） containing 10mM Tris pH-7.4, 50mM sodium chloride ａnd 1mM b-mercaptoethanol.
Solubility	It is recommended to reconstitute the lyophilized CABP29K in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized CABP29K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP29K should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 90% as determined by SDS-PAGE.
Applications	This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection. For adsorption we suggest the following procedure A- Dilute 1 μl of the antiserum against calretinin in 5 ml of the usual buffer for immunohistochemistry （final dilution 1:5＇000）. B- Add 1μg of recombinant protein to 1ml of the diluted antibody solution ａnd mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections ａnd incubate for 3 days. E - Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）. As a result, the immunostaining should be strongly reduced or even completely prevented.
Usage	Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.