Hieff NGS® Complete Adapter Kit for MGI®
产品信息
产品名称 | 产品编号 | 规格 | 价格（元） |
Hieff NGS® Complete Adapter Kit for MGI®, Set 1 | 13360ES04 | 16×4 T | 1153.00 |
13360ES16 | 16×16 T | 3653.00 |
13360ES96 | 16×100T | 15653.00 |
Hieff NGS® Complete Adapter Kit for MGI®, Set 2 | 13361ES04 | 16×4 T | 1153.00 |
13361ES16 | 16×16 T | 3653.00 |
13361ES96 | 16×100T | 15653.00 |
Hieff NGS® Complete Adapter Kit for MGI®, Set 3 | 13362ES04 | 16×4 T | 1153.00 |
13362ES16 | 16×16 T | 3653.00 |
13362ES96 | 16×100T | 15653.00 |
Hieff NGS® Complete Adapter Kit for MGI®, Set 4 | 13363ES04 | 16×4 T | 1153.00 |
13363ES16 | 16×16 T | 3653.00 |
13363ES96 | 16×100T | 15653.00 |
Hieff NGS® Complete Adapter Kit for MGI®, Set 5 | 13364ES04 | 16×4 T | 1153.00 |
13364ES16 | 16×16 T | 3653.00 |
13364ES96 | 16×100T | 15653.00 |
Hieff NGS® Complete Adapter Kit for MGI®, Set 6 | 13365ES04 | 16×4 T | 1153.00 |
13365ES16 | 16×16 T | 3653.00 |
13365ES96 | 16×100T | 15653.00 |
产品描述
Hieff NGS® Complete Adapter Kit for MGI®是针对MGI高通量文库构建专用配套试剂盒，共分为6个Set，每个Set中包含16种不同的Barcode接头，适用于MGI高通量测序平台多样品DNA文库构建。试剂盒中提供的所有试剂都经过严格的质量控制和功能验证，Zda程度保证了文库构建的稳定性和可重复性。
产品组分
编号 | 组分 | 产品规格 |
16×4 T | 16×16 T | 16×100 T |
13360 | DNA Adapter 1-16 | 20 μL each | 80 μL each | 500μL each |
13361 | DNA Adapter 17-32 | 20 μL each | 80 μL each | 500μL each |
13362 | DNA Adapter 33-48 | 20 μL each | 80 μL each | 500μL each |
13363 | DNA Adapter 49-64 | 20 μL each | 80 μL each | 500μL each |
13364 | DNA Adapter 65-80 | 20 μL each | 80 μL each | 500μL each |
13365 | DNA Adapter 81-96 | 20 μL each | 80 μL each | 500μL each |
运输和保存方法
冰袋运输，-20℃保存，有效期1年。
注意事项
1）本试剂盒中DNA Adapter的浓度统一为10 μM，单个文库构建的接头使用量根据所用试剂盒进行调整。
2）若有使用 MGI 其它建库试剂盒中的序号为 501-596 的接头，由于设计工艺不同，禁止混用，否则数据无法拆分。
3）切勿加热接头，应在室温让其缓慢溶解，实验室温度Z好设置为20-25℃。接头避免反复冻融，可短暂存放于4℃。
4）小规格试剂盒（16×4 T）每种组分的DNA Adapter包装量足够进行64个DNA文库构建，中规格试剂盒（16×16 T）每种组分的DNA Adapter包装量足够进行96个DNA文库构建，大规格试剂盒（16×100 T）每种组分的DNA Adapter包装量足够进行1600个DNA文库构建。
5）使用Hieff NGS® Complete Adapter Kit for MGI®试剂盒构建的DNA文库结构如下：
5’ - Ad153Ω_Bottom_ 2 - Insert DNA Sequence - Ad153_5T_1-index- 3’
6）试剂盒中提供的每种DNA Adapter中都包含Ad153_5T_1-index，且提供一种Barcode序列标签，用于高通量测序时区分样品。
7）为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用方法
DNA Adapter的序列如下：
Ad153Ω_Bottom_ 2：5’-TTGTCTTCCTAAGGAACGACATGGCTACGATCCGACTT-3’
Ad153_5T_1-index：5’-AGTCGGAGGCCAAGCGGTCTTAGGAAGACAANNNNNNNNNN1CAACTCCTTGGCTCACA-3’
1NNNNNNNNNN表示接头的Barcode区域，其在每个接头中的序列见下表。测序前在Sample Sheet中只需输入这个Barcode序列（10 bp）即可。
名称 | 序列 | | 名称 | 序列 |
Ad153_5T_1-index1 | TAGGTCCGAT | | Ad153_5T_1-index49 | ATGTACGCAG |
Ad153_5T_1-index2 | GGACGGAATC | | Ad153_5T_1-index50 | TTAATGAGAT |
Ad153_5T_1-index3 | CTTACTGCCG | | Ad153_5T_1-index51 | TGCGCCACTT |
Ad153_5T_1-index4 | ACCTAATTGA | | Ad153_5T_1-index52 | CATTAAGGCC |
Ad153_5T_1-index5 | TTCGTATCCG | | Ad153_5T_1-index53 | CCGCCTCAGA |
Ad153_5T_1-index6 | GGTAACGAGC | | Ad153_5T_1-index54 | AATCGGCTCG |
Ad153_5T_1-index7 | CAACGTATAA | | Ad153_5T_1-index55 | GCCGGTTATC |
Ad153_5T_1-index8 | ACGTCGCGTT | | Ad153_5T_1-index56 | GGAATATTGA |
Ad153_5T_1-index9 | TTCTGCTAGC | | Ad153_5T_1-index57 | ATTCAACGGA |
Ad153_5T_1-index10 | AGGAAGATAG | | Ad153_5T_1-index58 | AACTGTACTG |
Ad153_5T_1-index11 | GCTCTTGCTT | | Ad153_5T_1-index59 | GTACCTCAAT |
Ad153_5T_1-index12 | CAAGCACGCA | | Ad153_5T_1-index60 | GACTTCTAAT |
Ad153_5T_1-index13 | CGGCAATCCG | | Ad153_5T_1-index61 | TGAAGCGTTG |
Ad153_5T_1-index14 | ATCAGGATTC | | Ad153_5T_1-index62 | CGTGCGATCC |
Ad153_5T_1-index15 | TCATTCCAGA | | Ad153_5T_1-index63 | TCGGAAGGCA |
Ad153_5T_1-index16 | GATGCTGGAT | | Ad153_5T_1-index64 | CCGATGTCGC |
Ad153_5T_1-index17 | GTGAGTGATG | | Ad153_5T_1-index65 | ACTTAGAATG |
Ad153_5T_1-index18 | GAGTCAGCTG | | Ad153_5T_1-index66 | TCCAAGCCTG |
Ad153_5T_1-index19 | TGTCTGCGAA | | Ad153_5T_1-index67 | AGACGATGAT |
Ad153_5T_1-index20 | ATTGGTACAA | | Ad153_5T_1-index68 | CTCACAAGAC |
Ad153_5T_1-index21 | CGATTGTGGT | | Ad153_5T_1-index69 | CGTTCCTACT |
Ad153_5T_1-index22 | ACAGACTTCC | | Ad153_5T_1-index70 | GTGGTTGTGA |
Ad153_5T_1-index23 | TCCACACTCT | | Ad153_5T_1-index71 | GAAGGCCTGC |
Ad153_5T_1-index24 | CACCACAAGC | | Ad153_5T_1-index72 | TAGCTTGCCA |
Ad153_5T_1-index25 | TAGAGGACAA | | Ad153_5T_1-index73 | GACAATGCTC |
Ad153_5T_1-index26 | CCTAGCGAAT | | Ad153_5T_1-index74 | GCTAATCACA |
Ad153_5T_1-index27 | GTAGTCATCG | | Ad153_5T_1-index75 | AGTCCATAGG |
Ad153_5T_1-index28 | GCTGAGCTGT | | Ad153_5T_1-index76 | CTATCGCCTA |
Ad153_5T_1-index29 | AACCTAGATA | | Ad153_5T_1-index77 | ATCGTGGTCT |
Ad153_5T_1-index30 | TTGCCATCTC | | Ad153_5T_1-index78 | TGGCTAATAC |
Ad153_5T_1-index31 | AGATCTTGCG | | Ad153_5T_1-index79 | CAGTGCAGAG |
Ad153_5T_1-index32 | CGCTATCGGC | | Ad153_5T_1-index80 | TCAGGCTGGT |
Ad153_5T_1-index33 | GCAACGATGG | | Ad153_5T_1-index81 | ATACTCACGC |
Ad153_5T_1-index34 | TAATCGTTCA | | Ad153_5T_1-index82 | ATGCTCCGCG |
Ad153_5T_1-index35 | GTTCGCTCTA | | Ad153_5T_1-index83 | TGTGAACTTG |
Ad153_5T_1-index36 | TCTCACACAT | | Ad153_5T_1-index84 | GAGAGGTGCT |
Ad153_5T_1-index37 | CTGTTAGGAT | | Ad153_5T_1-index85 | TGCACTGTAA |
Ad153_5T_1-index38 | CGCAGACGCG | | Ad153_5T_1-index86 | GCCTAGGCAA |
Ad153_5T_1-index39 | AAGGATCATC | | Ad153_5T_1-index87 | CCATCATAGC |
Ad153_5T_1-index40 | AGCGTTGAGC | | Ad153_5T_1-index88 | CATGGTAATT |
Ad153_5T_1-index41 | TTAGATGCAT | | Ad153_5T_1-index89 | CACCATGTCT |
Ad153_5T_1-index42 | GTCCAGAGCT | | Ad153_5T_1-index90 | ATATGTCTGG |
Ad153_5T_1-index43 | CACGTGATAG | | Ad153_5T_1-index91 | AAGGAAGCGT |
Ad153_5T_1-index44 | CCACTAGTCC | | Ad153_5T_1-index92 | TCAAGACGTC |
Ad153_5T_1-index45 | TGGACTTGGC | | Ad153_5T_1-index93 | CCGCTCAGTA |
Ad153_5T_1-index46 | GCTTGACAGG | | Ad153_5T_1-index94 | GGTGTGTACA |
Ad153_5T_1-index47 | AAGACCTCTA | | Ad153_5T_1-index95 | TTCACGTAAG |
Ad153_5T_1-index48 | AGTTGCCATA | | Ad153_5T_1-index96 | GGTTCCACAC |
推荐Barcode组合：
4个Adapter 成组：1-4,13-16；
8个Adapter 成组：41-48。
相关产品
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Hieff NGS® Ultima DNA Library Prep Kit for MGI® | 13310ES16/96 | 16/96 T | 4763/24563 |
Hieff NGS® OnePot II DNA Library Prep Kit for MGI® | 13321ES16/96 | 16/96 T | 5363/26563 |
Hieff NGS® MaxUp II Dual-mode mRNA Library Prep Kit for MGI® | 13331ES16/96 | 16/96 T | 4563/22563 |
文库构建磁珠 | 产品编号 | 规格 | 价格（元） |
Hieff NGS® cfDNA Clean Beads（100~200 bp） | 12599ES08/56 | 5/60 mL | 1666/9106 |
Hieff NGS® Smarter DNA Clean beads (50 bp以上） | 12600ES08/56 | 5/60 mL | 1386/7586 |
Hieff NGS® DNA Selection Beads（Superior AMPure XP alternative） | 12601ES08/56 | 5/60 mL | 986/6286 |
Hieff NGS® mRNA Isolation Master Kit | 12603ES24/96 | 24/96 T | 616/2266 |
建库接头 | 产品编号 | 规格 | 价格（元） |
Hieff NGS® Complete Adapter Kit for MGI, Set 1 | 13360ES04/16/96 | 16×4/×16/×100 T | 1153/3653/15653 |
Hieff NGS® Complete Adapter Kit for MGI, Set 2 | 13361ES04/16/96 | 16×4/×16/×100 T | 1153/3653/15653 |
Hieff NGS® Complete Adapter Kit for MGI, Set 3 | 13362ES04/16/96 | 16×4/×16/×100 T | 1153/3653/15653 |
Hieff NGS® Complete Adapter Kit for MGI, Set 4 | 13363ES04/16/96 | 16×4/×16/×100 T | 1153/3653/15653 |
Hieff NGS® Complete Adapter Kit for MGI, Set 5 | 13364ES04/16/96 | 16×4/×16/×100 T | 1153/3653/15653 |
Hieff NGS® Complete Adapter Kit for MGI, Set 6 | 13365ES04/16/96 | 16×4/×16/×100 T | 1153/3653/15653 |
建库模块 | 产品编号 | 规格 | 价格（元） |
Hieff NGS® Fast-PaceTM DNA Cyclization Kit for MGI® | 13341ES16/96 | 16/96 T | 1853/9253 |
Hieff NGS® Fast-Pace DNA Ligation Module | 12607ES24/96 | 24/96 T | 3263/10863 |
Hieff NGS® Fast-Pace End Repair/dA-Tailing Module | 12608ES24/96 | 24/96 T | 2066/6166 |
2×Super Canace®Ⅱ High-Fidelity Mix for Library Amplification | 12621ES24/96 | 24/96 T | 583/1783 |
Hieff NGS® Dual-Mode cDNA Synthesis Kit | 12250ES24/96 | 24/96 T | 4885/16485 |
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Hieff NGS® Library Quantification Kit for Illumina®, DNA Standard (1-6) | 12307ES09 | 6×96 μL | 2126 |
dsDNA HS Assay Kit for Qubit® | 12640ES60/76 | 100/500 T | 686/2696 |
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