SnapFast Vectors Sigma-Aldrich 代谢组学克隆和表达_详细资料

产品信息

品牌 sigma 货号 SnapFast? Vectors克隆和表达规格基因组编辑工具供货周期一周主要用途代谢组学

基因组编辑工具-CRISPR/Cas9
继ZFN（Zinc Finger Nucleases）技术后，Merck在2013年推出新一代基因组编辑工具--CRISPR/Cas9，让研究人员以更快、更经济的方式实现基因组特定位点的编辑。凭借过去10年在基因组编辑领域的丰富经验积累以及专业的生物信息学平台，Merck已经成功设计出覆盖人类，小鼠和大鼠三个物种的所有基因的CRISPR/Cas9载体，并可以提供在线定制服务，以及完整的CRISPR实验workflow解决方案。此外，默克与Sanger Institute合作开发了人、小鼠全基因组CRISPR 文库，以帮助科学家实现基因功能的快速筛选、规模化的模型建立以及药物作用筛选等。
GX：优化的载体设计，限度提高转染效率，简化筛选工作
特异：特殊的gRNA设计和双切口酶系统，限度提高特异性
全面：产品齐全，可提供质粒、RNA、慢病毒载体、RNP等形式，涵盖人、大鼠、小鼠、植物等多个物种，更有Sanger Arrayed和Broad Pools全基因组文库以及重要通路的亚文库
掌控：强大的慢病毒全基因组文库可轻松进行高通量筛选，全面掌控人或小鼠的基因组

CRISPR实验--载体构建和表达
• CRISPR/Cas gRNA设计和选择
• crRNA定制合成
• 克隆和表达
• 抗生素
• GenElute™质粒DNA快速提取kit
• PCR扩增和核酸电泳
• SP6/T7 体外转录试剂盒
• GenElute™总RNA提取试剂盒
• 慢病毒包装系统
• 培养皿/离心管/枪头等基础耗材
Molecular BiologyCloning & Expression
The aim of molecular cloning is to insert the gene-of-interest (GOI) into a plasmid vector. This vector is then inserted into a cell that will express the protein encoded by the GOI. Once protein is expressed, the protein function can be studied as it affects the cell signaling, morphology or other aspects. Alternatively, the protein can be expressed in large quantities that can then be studied directly with other techniques. We have a wide variety of products for use across the cloning & expression workflow.
expression workflow.

Cloning Products
• SnapFast™ Vectors
• Cloning Enzymes
• Restriction Enzymes
• Biomatrica™ Nucleic Acid Stabilization
• Custom DNA Oligos
Special OfferStandardized Cloning Vectors using SnapFast™ Technology
Traditional cloning by restriction enzyme digestion remains the most popular way to insert your gene-of-interest (GOI) into an expression vector for expression in the target cell, whether that is an insect, mammalian, or microbial cell. This cloning method is easy, reliable, and cost-conscious, but lacks standardization due to labs using a variety of different plasmid backbones.
To answer the need of standardization, Oxford Genetics uses patented SnapFast™ Technology to make genetic engineering cheaper and more efficient by providing scientists with a collection of versatile cloning vectors, and a protocol to easily move the GOI from one vector to another. The vectors can be used with any cloning system you like: traditional restriction enzyme cloning, Gibson Assembly^®, LIC, GeneArt^®, and InfusionHD.
The concept is simple: One plasmid, a thousand possibilities. All of the DNA plasmids are based on the same core backbone - the SnapFast vector. All of the DNA sections or components within the SnapFast system are designed to be fully inter-changeable, so you can move your GOI from one vector to another with ease. You can express your gene with a different reporter gene, with a different tag, or in a different species by simply moving the GOI into a different vector, using the SnapFast cutting sites in one fast & easy protocol.
Our most popular cloning vectors and vector sets are listed below, but the collection gives you choice of:
26 peptide tags
9 reporter genes
>20 signal peptides
>40 promoters
10 selection markers
Browse hundreds of options easily using our NEW SELECTION GUIDE or ENHANCED SEARCHING.

Plasmid Product Name Product No. Application
NEW SnapFast™ Vectors based on the most popular pFLAG Vectors
pSF-CMV-NEO-NH2-PPT-3XFLAG OGS626 CMV promoter with strong expression
pSF-CMV-NEO-NH2-3XFLAG OGS627 CMV promoter with strong expression
pSF-CMV-NEO-COOH-3XFLAG OGS629 CMV promoter with strong expression
Most Popular Bacterial Reporter Genes
pSF-OXB20-BetaGal OGS207 RecA promoter driving b-galactosidase
pSF-OXB20-Fluc OGS412 OXB20 promoter driving firefly luciferase
Most Popular Bacterial Promoters
pSF-OXB20 OGS50 OXB20 promoter (strongest expression)
pSF-OXB1 OGS553 OXB1 promoter (weakest expression)
pSF-Tac OGS501 Tac promoter (IPTG inducible expression)
pSF-LacI OGS502 LacI promoter (regulated expression)
Most Popular Mammalian Reporter Genes
pSF-MINCMV-DAGFP OGS573 Minimal CMV promoter for low expression
pSF-CMV-FMDV-daGFP OGS289 Dual promoters driving IRES daGFP
pSF-CMV-RSV-SEAP AscI OGS17 Dual promoters driving SEAP
Most Popular Mammalian Promoters
pSF-SYN1 OGS503 Synapsin 1 neuron-specific promoter
pSF-CAG-AMP OGS504 CAG promoter strong and stable expression
pSF-CMV-AMP OGS2 CMV promoter strongest expression
pSF-CAG-UB-PURO OGS600 CAG promoter strong and stable expression
pSF-CMV-Kan OGS10 CMV promoter (strongest expression)
pSF-CAG-Kan OGS505 Synthetic CAG promoter (strong and stable expression)
pSF-EF1 Alpha OGS43 EF1a promoter (moderate but stable expression)
Most Popular Mammalian Selection Markers
pSF-CMV-PGK-Puro OGS394 Dual promoter for expressing two genes (puromycin)
pSF-CMV-FMDV-Hygro OGS292 Dual promoter expressing one gene (hygromycin)
pSF-CMV-Blast OGS588 CMV promoter (blasticidin)
pSF-CMV-Ub-Neo/G418 AscI OGS22 Dual promoter expressing one gene (G418)
Most Popular Mammalian Protein Tags
pSF-CMV-PURO-NH2-GST-TEV OGS1129 N-term GST tag & TEV cleavage tag
pSF-CMV-PURO-COOH-TEV-GST OGS1130 C-term GST tag & TEV cleavage tag
pSF-CMV-PURO-NH2-CMYC OGS3214 N-term C-myc tag
pSF-CMV-Puro-COOH-TEV-FLAG^®-6His OGS1232 C-term FLAG^®, 6His affinity tags & TEV cleavage tag
Most Popular Bacterial Protein Tags
pSF-OXB20-NH2-10His-EKT OGS2806 N-term 10His affinity tag & EKT cleavage tag
pSF-OXB20-NH2-FLAG(R)-6His-EKT OGS2828 N-term FLAG^®, 6His affinity tags & EKT cleavage tag
pSF-OXB20-COOH-TEV-10His OGS2767 C-term 10His affinity tag & TEV cleavage tag
pSF-OXB20-COOH-TEV-FLAG(R)-6His OGS2891 C-term FLAG^®, 6His affinity tags & TEV cleavage tag
Most Popular Yeast Protein Tags
pSF-TEF1-NH2-10His-EKT OGS1649 N-term 10His affinity tag & EKT cleavage tag
pSF-TEF1-NH2-FLAG^®-6His-EKT OGS1658 N-term FLAG^®, 6His affinity tags & EKT cleavage tag
pSF-TEF1-COOH-TEV-10His OGS1913 C-term 10His affinity tag & TEV cleavage tag
pSF-TEF1-COOH-TEV-FLAG^®-6His OGS1969 C-term FLAG^®, 6His affinity tags & TEV cleavage tag
Other Popular Plasmids
pSF-CMV-CRE OGS591 CRE recombinase for LoxP excision
PUC19 OGS590 industry standard high copy number cloning vector
PBR322 OGS589 industry standard low copy number cloning vector
pSF-RENILLA-PHOTINUS OGS595 bidrectional expression of two luciferase reporters
pSF-CMV-HUIGG1 HC OGS527 seamless fusion of variable antibody fragments
pSF-TEFI-TPI1-Fluc-URA3 OGS545 Yeast promoter driving firefly luciferase (plus URA3 selection)
pSF-CMV-CMV-SbfI-Ub-Puro OGS597 Dual CMV promoters for expressing two genes
Popular Plasmid Sets
Mammalian Signal Peptide Pack PP2379 Eight plasmids to optimize signal peptide use
Yeast Promoter Vector Pack PP2357 Five plasmids to optimize expression based on promoter strength
GFP Vector Pack PP2368 Five plasmids to optimize the configuration of the GFP reporter gene
Mammalian Promoters Pack PP2356 Six plasmids to optimize expression based on promoter strength

GeneArt is owned by Life Technologies.
Gibson Assembly is owned by Synthetic Genomics.
SnapFast is owned by Oxford Genetics.

您可能感兴趣的产品