Phospho-Serine Antibody, Biotin | |
Catalog #: | I03 |
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Product Description | The affinity purified rabbit anti-phospho-serine antibodies are conjugated to d-biotin. The conjugates are useful for detection, identification, and quantization of serine-phosphorylated proteins and peptides. | |
Species | Rabbit | |
Formulation | 250 µg/mL in PBS, 40% glycerol | |
Immunogen | Phospho-serine-KLH conjugates |
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Purification | The antibody was purified with phospho-serine on agarose |
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Conjugation | Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin) |
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Specificity | The antibody is pan-specific against phospho-serine. It does not cross react with phospho-tyrosine but does react slightly with phospho-threonine. |
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Applications | ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL) |
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Scientific Description | Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to form phospho-serine, which is responsible for various signalling processes in eukaryotes. [1] |
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Storage & Stability |
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored. |
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Product Specific References | 1. Curr. Opin. Cell. Bio. 2001. 13(2): 131-138. doi:10.1016/S0955-0674(00)00189-7
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Phospho-Threonine Antibody, Biotin | |
Catalog #: | ICP9820 |
Product Description | Affinity purified rabbit anti-phospho-threonine antibodies are conjugated to d-biotin via N-acetylation. The conjugates are useful for detection, identification and quantization of threonine-phosphorylated proteins and peptides. |
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Species | Rabbit
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Formulation | 250 µg/mL in PBS, 50% glycerol
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Immunogen | Phospho-threonine-KLH conjugates and phosvitin
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Purification | The antibody was purified on phospho-threonine agarose.
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Conjugation | Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin)
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Specificity | The antibody is pan-specific against phospho-threonine. It cross reacts with phospho-serine slightly but does not cross-react with phospho-tyrosine.
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Applications | ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)
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Scientific Description | Threonine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate threonine to form phospho-threonine which is considered a key step in the regulation of enzymatic activities such as signal transduction.
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Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.
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Product Specific References |
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Phospho-Tyrosine Antibody, HRP | |
Catalog #: | I02 |
Product Description | The affinity purified rabbit polyclonal anti-phospho-tyrosine antibody was conjugated to horseradish peroxidase (HRP). The conjugates are useful for detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides. |
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Species | Rabbit | ||
Formulation | 250 µg/mL in HRP stabilizing buffer with 50% glycerol | ||
Immunogen | Iodoacetylphosphotyramine-KLH conjugates | ||
Purification | The antibody was affinity purified with phospho-tyrosine immobilized on agarose, followed by immune affinity absorption with tyrosine on agarose. | ||
Conjugation | Reductive amination; HRP/Ab ratio - 10:1 | ||
Specificity | The antibody is pan-specific against phospho-tyrosine. There are no cross reactions with phospho-serine or phospho-threonine. | ||
Applications | ELISA (1:1000); WB (1:1000) | ||
Scientific Description | Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to form phospho-tyrosine which is considered a key step in the regulation of enzymatic activities such as signal transduction.
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Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. | ||
Product Specific References |
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