Methyl Mono-, Di-Lysine Antibody

Description: Affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a unique technique of ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with methylated lysine residues
Species: Rabbit

Formulation: 250 µg/mL in PBS with 50% glycerol.

Storage & Stability: Store product at -20℃. One year from date of shipping.

Immunogen: Methylated KLH conjugates.

Purification: The antibody was immunoaffinity purified with epsilon N-methyl lysine on agarose.

Specificity:  The antibody recognizes proteins or peptides methylated on lysine residues (mono, di-methylysine). There is no cross-reaction with acetylated protein. The cross-reactivity with tri-methyllysine has not been tested.

Conjugation: Reductive amination

Application(s): ELISA, western blot, immunohistochemistry.

Scientific Description: Methylation on the lysine residue of protein (MeK) is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3]

References: 1. Im, H. et al. J. Biol. Chem. 2003, 278, 18346-18352. 2. Ng, H. H. et al. J. Biol. Chem. 2002, 277, 34655-34657. 3. Zegerman, P. et al. J. Biol. Chem. 2002, 277, 11621-11624.