部分发表文献: O.Guillaume-Gentil, E.Potthoff, D.Ossola, et al. Force-controlled fluidic injection into single cell nuclei.(2013)Small,9(11),1904−1907. doi:10.1002/ smll.201202276A. Meister, M. Gabi, P.Behr, et al. FluidFM: Combining atomic force microscopy and nanofluidics in a universal liquid delivery system for single cell applications and beyond.(2009) Nano Letters, 9(6), 2501−2507. doi:10.1021/nl901384x |
单细胞提取——微量、低创、JZ | | 活细胞提取 从活细胞中直接提取内容物,并且提取后细胞仍可存活。 | | | 电镜成像 相比于传统的裂解方式,FluidFM BOT提取的样本更为干净,可以得到很好地电镜图像。 | | | mRNA、酶活力的检测 FluidFM BOT提取的样本也可以直接用于酶活力的测定或mRNA的检测。 | | | 单细胞质谱分析 FluidFM BOT提取样本也可应用于单细胞代谢组学样本的质谱分析。 |
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部分发表文献: O. Guillaume-Gentil, T. Rey, P. Kiefer, A.J. Ibáñez, R. Steinhoff, R. Brönnimann, L. Dorwling-Carter, H. Zambelli, R. Zenobi & J.A. Vorholt. Single-Cell Mass Spectrometry of Metabolites Extracted from Live Cells by Fluidic Force Microscopy. (May 2017) Anal Chem., 89(9), 5017-5023. doi:10.1021/acs.analchem.7b00367 O. Guillaume-Gentil, R.V. Grindberg, R. Kooger, L. Dorwling-Carter, V. Martinez, D. Ossola, M. Pilhofer, T. Zambelli & J.A. Vorholt. Tunable Single-Cell Extraction for Molecular Analyses. (Jul 2016) Cell, 166(2), 506-516. doi: 10.1016/j. cell.2016.06.025. |
单细胞分离——直观、简单、低损 |
| | FluidFM® 一气呵成的细胞分离过程 使用FluidFM BOT分离CHO细胞,仅仅点击几次鼠标,单个细胞便JZ的完成了转移。 |
| | 小样本细胞群分离的选择 对于细胞数不足以使用流式细胞仪分选时,FluidFM BOT很好的填补这个空白。 |
部分发表文献: O. Guillaume-Gentil, T. Zambelli & J.A. Vorholt.Isolation of single mammalian cells from adherent cultures by fluidic force microscopy. (2014) Lab on a chip, 14(2), 402-414. doi:10.1039/c3lc51174j P. Stiefel, T. Zambelli & J.A. Vorholt. Isolation of optically targeted single bacteria by application of fluidic force microscopy to aerobic anoxygenic phototrophs from the phyllosphere. (2013) Applied and Environmental Microbiology, 79(16), 4895-4905. doi:10.1128/AEM.01087-13P. Dörig, P. Stiefel, P. Behr, et al. Force-controlled spatial manipulation of viable mammalian cells and micro-organisms by means of FluidFM technology.(2010) Applied Physics Letters, 97(2), 023701 1-3. doi:10.1063/1.3462979 |