Anti-Homez抗体,同源盒亮氨酸拉链蛋白HOMEZ抗体产品介绍:Homeodomain-containing proteins function as transcription factors that typically switch on cascades of other genes. Usually homeodomain proteins act in the promoter region of their target genes as complexes with other transcription factors, leading to much higher target specificity than a single homeodomain protein. HOMEZ (Homeobox and leucine zipper protein) is a 525 amino acid nuclear protein that contains 3 atypical homeodomains, 2 leucine zipper-like motifs, proline and serine-rich motifs and an acidic domain. Within homeodomain 2, it contains a putative nuclear localization signal. HOMEZ shares significant sequence similarity with mouse ZHX1 and sequences that are homologous to HOMEZ are restricted to vertebrates. Likely functioning as a transcription regulator, HOMEZ is ubiquitously expressed with highest levels found in in ovary, testis, kidney, fetal lung and kidney.
简单介绍:
中文名称:同源盒亮氨酸拉链蛋白HOMEZ抗体
英文名称:Anti-Homez antibody
产品别名:Homeobox and leucine zipper protein Homez; Homeodomain leucine zipper containing factor; KIAA1443.
产品规格:0.1ml、0.2ml、1ml
产品用途:科研实验
产品价格:请报价
Anti-Homez抗体说明书:请添加客服直接索取
抗体来源:该指标有两种产品,一种是兔来源抗体,一种是鼠来源抗体
克隆类型:兔来源为多克隆抗体,鼠来源单克隆抗体
交叉反应:请索取说明书查看
性 状:Lyophilized or Liquid
浓 度:1mg/1ml
亚 型:IgG
纯化方法: affinity purified by Protein A
储 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
同源盒亮氨酸拉链蛋白HOMEZ抗体保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Homez抗体,同源盒亮氨酸拉链蛋白HOMEZ抗体实验步骤概述
1、 组织取材:组织块称重
2、 利用液氮、研钵粉碎组织块
3、 加入RIPA缓冲液(每克组织3ml RIPA),PMSF(每克组织30μl,10 mg/ml PMSF),
利用Polytron进一步匀浆(15,000转/分*1分钟)维持4℃
4、 加入PMSF(每克组织30μl,10 mg/ml PMSF),冰上孵育30分钟
5、 移入离心管4℃,约20,000g(约15,000转)15分钟
6、 上清液为细胞裂解液可分装-20℃保存
7、 进行Bradford比色法测定蛋白质浓度
8、 取相同质量的细胞裂解液(体积*蛋白质浓度),并加等体积的2×电泳加样缓冲液
9、 沸水浴中3分钟
10、上样
11、电泳(浓缩胶20mA,分离胶35mA)
12、电转膜仪转膜(100mA 40分钟)
13、膜用丽春红染色,胶用考马斯亮蓝染色
14、Westernblot 试剂盒显色
15、分析比较记录
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