Anti-acetyl Lysine抗体,乙酰基赖氨酸抗体

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Anti-acetyl Lysine抗体,乙酰基赖氨酸抗体别名：Ac-lysine; acetyl Lysine; Lysine.                        

中文名称：乙酰基赖氨酸抗体

英文名称：Anti-acetyl Lysine antibody

规格：0.1ml/0.2ml

Anti-acetyl Lysine抗体相关标记：Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC.

浓    度：1mg/1ml

抗体来源：Rabbit or Mouse or Goat 

克隆类型：polyclonal or monoclonal

产品类型：一抗

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

乙酰基赖氨酸抗体应用：可以用于做石蜡切片免疫组化，冰冻切片免疫组化，Elisa，WB，免疫荧光、流式细胞术等实验。

抗体修FF式

修复液：0.01M 枸橼酸（pH6.0）或者0.05M EDTA（pH8.0）

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Anti-acetyl Lysine抗体,乙酰基赖氨酸抗体内参即是内部参照（Internal Control），对于哺乳动物细胞表达来说一般是指由管家基因编码表达的蛋白(Housekeeping Proteins)，它们在各组织和细胞中的表达相对恒定，在检测蛋白的表达水平变化时常用它来做参照物。在Western Blotting 实验中，除了需要进行蛋白抽提、蛋白定量、等量蛋白上样电泳、转膜、靶蛋白抗体孵育、显色等步骤以外，还需要进行内参的检测，以校正蛋白质定量、上样过程中存在的实验误差，以保证实验结果的准确性。

实验中出现的问题及处理办法

1、为什么带出现拖尾现象？

原因：主要是样品融解效果不佳或分离胶浓度过大引起的；

处理办法：加样前离心，选择适当的样品缓冲液，加适量样品促溶剂；电泳缓冲液时间过长，重新配制;降低凝胶浓度。

2、为什么带出现纹理现象？

原因：主要是样品不溶性颗粒引起的；

处理办法：加样前离心，加适量样品促溶剂。

3、为什么电泳的条带很粗？

原因：电泳中条带很粗是常见的事，主要是未浓缩好的原因；

处理办法：适当增加浓缩胶的长度，保证浓缩胶贮液的pH正确（6.7），适当降低电压。

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Anti-acetyl Lysine抗体产品介绍：In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

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