V010018 必拓生物pCLDC载体，pCLDC vector，质粒载体图谱

V010018必拓生物pCLDC载体，pCLDC vector，质粒载体图谱

成分：10 mM Tris（pH8.0）

保存条件：-20℃保存

产品目录

pCLDC载体，pCLDC vector，质粒载体图谱

LOCUS       pCLDC        4377 bp    DNA     circular SYN 17-NOV-2016

FEATURES             Location/Qualifiers

     source          1..4377

                     /organism="Expression vector pColdIII"

                     /mol_type="other DNA"

                     /db_xref="taxon:289231"

                     /lab_host="Escherichia coli"

                     /vntifkey="98"

                     /note="expresses an open reading frame as a fusion to the translation enhancing element. This plasmid is derived from pUC118."

     gene            15..482

                     /gene="cspA"

                     /vntifkey="60"

     promoter        15..81

                     /gene="cspA"

                     /vntifkey="30"

                     /label=cspApromoter

     enhancer        256..270

                     /gene="cspA"

                     /vntifkey="9"

                     /label=cspATEE

                     /note="TEE (translation enhancing element)"

     gene            1341..2201

                     /gene="Amp"

                     /vntifkey="60"

     CDS             1341..2201

                     /gene="Amp"

                     /codon_start=1

                     /transl_table=11

                     /product="beta-lactamase"

                     /protein_id="BAD35138.1"

                     /db_xref="GI:51090254"

                     /vntifkey="4"

                     /label=Amp

     rep_origin      2361..2975

                     /vntifkey="33"

                     /label=ColE1Origin

                     /note="ColE1 origin"

     gene            complement(3132..4214)

                     /gene="lacI"

                     /vntifkey="60"

     CDS             complement(3132..4214)

                     /gene="lacI"

                     /codon_start=1

                     /transl_table=11

                     /product="lactose operon repressor"

                     /protein_id="BAD35139.1"

                     /db_xref="GI:51090255"

                     /vntifkey="4"

                     /label=lacI

BASE COUNT     1102 a      1079 c      1139 g      1057 t

ORIGIN

        1 aaggaatggt gtggccgatt aatcataaat atgaaaaata attgttgcat cacccgccaa

       61 tgcgtggctt aatgcacatc aaattgtgag cggataacaa tttgatgtgc tagcgcatat

      121 ccagtgtagt aaggcaagtc ccttcaagag ttatcgttga tacccctcgt agtgcacatt

      181 cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc gaaaggcaca cttaattatt

      241 aagaggtaat acaccatgaa tcacaaagtg catatggagc tcggtaccct cgagggatcc

      301 gaattcaagc ttgtcgacct gcagtctaga taggtaatct ctgcttaaaa gcacagaatc

      361 taagatccct gccatttggc ggggattttt ttatttgttt tcaggaaata aataatcgat

      421 cgcgtaataa aatctattat tatttttgtg aagaataaat ttgggtgcaa tgagaatgcg

      481 caggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct

      541 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg

      601 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat

      661 tgtactgaga gtgcaccata aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa

      721 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa

      781 atcaaaagaa tagcccgaga tagggttgag tgttgttcca gtttggaaca agagtccact

      841 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc

      901 actacgtgaa ccatcaccca aatcaagttt tttggggtcg aggtgccgta aagcactaaa

      961 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc

     1021 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt

     1081 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtacta

     1141 tggttgcttt gacgtatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc

     1201 atcaggcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt

     1261 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat

     1321 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt

     1381 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg

     1441 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga

     1501 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc

     1561 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac

     1621 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg

     1681 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca

     1741 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg

     1801 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg

     1861 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg

     1921 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag

     1981 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg

     2041 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct

     2101 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac

     2161 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact

     2221 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga

     2281 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt

     2341 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct

     2401 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc

     2461 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc

     2521 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc

     2581 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg

     2641 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt

     2701 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg

     2761 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg

     2821 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt

     2881 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag

     2941 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt

     3001 gctggccttt tgctcacata gtcatgcccc gcgcccaccg gaaggagctg actgggttga

     3061 aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa

     3121 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat

     3181 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt

     3241 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc

     3301 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac

     3361 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca

     3421 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg

     3481 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa

     3541 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg

     3601 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct

     3661 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg

     3721 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac

     3781 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag

     3841 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct

     3901 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga

     3961 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg

     4021 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc

     4081 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg

     4141 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac

     4201 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata

     4261 ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga

     4321 ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgc

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