V01016 必拓生物pBADk载体，pBADk vector，质粒载体图谱

V010016必拓生物pBADk载体，pBADk vector，质粒载体图谱

成分：10 mM Tris（pH8.0）

保存条件：-20℃保存

产品目录

pBADk载体，pBADk vector，质粒载体图谱

LOCUS       pBADk        5435 bp    DNA     circular SYN 17-NOV-2016

FEATURES             Location/Qualifiers

     source          1..5435

                     /organism="synthetic construct"

                     /mol_type="other DNA"

                     /db_xref="taxon:32630"

                     /lab_host="Escherichia coli"

                     /plasmid="pBAD24"

                     /vntifkey="98"

     gene            96..974

                     /gene="araC"

                     /vntifkey="60"

     mRNA            complement(96..974)

                     /gene="araC"

                     /vntifkey="54"

                     /label=araC

     rep_origin      4066..4763

                     /vntifkey="33"

                     /label=pBR322Origin

                     /note="pBR322 origin"

     promoter        1248..1277

                     /vntifkey="30"

                     /label=arapromoter

     misc_feature    1208..1227

                     /vntifkey="21"

                     /label=pBADfwdprimer

     misc_feature    complement(1395..1412)

                     /vntifkey="21"

                     /label=pTrcHisrevprimer(pBADrevprimer)

     terminator      1445..1602

                     /vntifkey="43"

                     /label=rrnB

     misc_feature    2310..3122

                     /vntifkey="21"

                     /label=Kan

BASE COUNT     1337 a      1379 c      1338 g      1381 t

ORIGIN

        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac

       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca

      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta

      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata

      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag

      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag

      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg

      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct

      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc

      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc

      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca

      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga

      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa

      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata

      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc

      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt

      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat

     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta

     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt

     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca

     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta

     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt

     1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga

     1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag

     1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg

     1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag

     1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt

     1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa

     1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa

     1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt

     1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat

     1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt

     1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg

     1981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga

     2041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc

     2101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac

     2161 actattctca gaatgacttg gttgagtggg ggggggggga aagccacgtt gtgtctcaaa

     2221 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg

     2281 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct

     2341 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg

     2401 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag

     2461 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca

     2521 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc

     2581 ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc caggtattag

     2641 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt

     2701 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc

     2761 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta

     2821 atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg

     2881 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat

     2941 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca

     3001 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat

     3061 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt

     3121 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg

     3181 acggcggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct

     3241 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac

     3301 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat

     3361 tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc gccccccccc

     3421 ccctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat

     3481 ctacacgacg gggagtcagg caactatgga tgacgaatag acagatcgct gagataggtg

     3541 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg

     3601 atttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg

     3661 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc

     3721 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga

     3781 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt

     3841 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat

     3901 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat

     3961 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa

     4021 tttaacgcga attttaacaa aatattaacg tttacaattt aaaaggatct aggtgaagat

     4081 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc

     4141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg

     4201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct

     4261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct

     4321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct

     4381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg

     4441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc

     4501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga

     4561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg

     4621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta

     4681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg

     4741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg

     4801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat

     4861 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc

     4921 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg

     4981 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag

     5041 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca

     5101 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct

     5161 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg

     5221 aggcagcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac

     5281 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc

     5341 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc

     5401 ggcgtagagg atctgctcat gtttgacagc ttatc

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