V01015 必拓生物pTac28载体，pTac28 vector，质粒载体图谱

V01015必拓生物pTac28载体，pTac28 vector，质粒载体图谱

成分：10 mM Tris（pH8.0）

保存条件：-20℃保存

产品目录

pTac28载体，pTac28 vector，质粒载体图谱

LOCUS       pTac28        5414 bp    DNA     circular     17-NOV-2016

COMMENT     This file is created by Vector NTI

            http://www.invitrogen。。ｃｏｍ/

FEATURES             Location/Qualifiers

     misc_feature    295..312

                     /vntifkey="21"

                     /label=His6

     misc_feature    complement(1014..1826)

                     /vntifkey="21"

                     /label=kan

     misc_feature    complement(3969..5048)

                     /vntifkey="21"

                     /label=lacI

     promoter        33..61

                     /vntifkey="30"

                     /label=tacpromoter

     misc_feature    165..182

                     /vntifkey="21"

                     /label=His6

     rep_origin      1872..3005

                     /vntifkey="33"

                     /label=ColE1Origin

BASE COUNT     1276 a      1456 c      1404 g      1278 t

ORIGIN

        1 agatctctgg caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg

       61 tgtggaattg tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt

      121 tcacgagcac ttcaccaaca aggaccatag ccatgggcag cagccatcat catcatcatc

      181 acagcagcgg cctggtgccg cgcggcagcc atatggctag catgactggt ggacagcaaa

      241 tgggtcgcgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac

      301 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct

      361 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt

      421 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg

      481 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc

      541 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc

      601 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg

      661 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg

      721 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg

      781 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt

      841 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa

      901 tattaacgtt tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt

      961 gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac

     1021 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt

     1081 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca

     1141 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc

     1201 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt

     1261 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc

     1321 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg

     1381 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg

     1441 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat

     1501 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta

     1561 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc

     1621 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc

     1681 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga

     1741 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa

     1801 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac

     1861 agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga

     1921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg

     1981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc

     2041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct

     2101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc

     2161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt

     2221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg

     2281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct

     2341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag

     2401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag

     2461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg

     2521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg

     2581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac

     2641 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt

     2701 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat

     2761 ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc

     2821 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa

     2881 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg

     2941 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga

     3001 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt

     3061 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc

     3121 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat

     3181 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta

     3241 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga

     3301 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg

     3361 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc

     3421 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg

     3481 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg

     3541 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca

     3601 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc

     3661 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga

     3721 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa

     3781 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa

     3841 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc

     3901 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt

     3961 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg

     4021 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc

     4081 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag

     4141 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg

     4201 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg

     4261 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc

     4321 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac

     4381 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat

     4441 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc

     4501 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca

     4561 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga

     4621 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg

     4681 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg

     4741 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta

     4801 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc

     4861 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc

     4921 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc

     4981 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact

     5041 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga

     5101 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg

     5161 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg

     5221 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac

     5281 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc

     5341 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc

     5401 ggcgtagagg atcg

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