V010011 必拓生物pRSETA载体，pRSETA vector，质粒载体图谱

V010011必拓生物pRSETA载体，pRSETA vector，质粒载体图谱

T7强启动子，高表达载体，可与pET系列部分载体替换使用

成分：10 mM Tris（pH8.0）

保存条件：-20℃保存

产品目录

必拓生物pRSETA载体，pRSETA vector，质粒载体图谱

LOCUS       pRSET-A        2897 bp    DNA     circular      2-MAY-2017

DEFINITION  pRSET-A.

SOURCE      

  ORGANISM  other sequences; artificial sequences; vectors.

FEATURES             Location/Qualifiers

     source          1..2897

                     /organism="pRSET-A"

                     /mol_type="other DNA"

                     /vntifkey="98"

     promoter        20..38

                     /vntifkey="30"

                     /label=T7_promoter

     misc_feature    75..91

                     /vntifkey="21"

                     /label=T7_transl_en_RBS

     misc_feature    132..150

                     /vntifkey="21"

                     /label=Xpress_fwd_primer

     misc_feature    169..192

                     /vntifkey="21"

                     /label=Xpress_EK

     gene            1042..1902

                     /gene="Ampicillin"

                     /vntifkey="60"

     CDS             1042..1902

                     /vntifkey="4"

                     /label=AmpR

     rep_origin      2057..2676

                     /vntifkey="33"

                     /label=pBR322_origin

BASE COUNT      721 a       707 c       727 g       742 t

ORIGIN

        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct

       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat

      121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac

      181 gatgacgata aggatcgatg gggatccgag ctcgagatct gcagctggta ccatggaatt

      241 cgaagcttga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg

      301 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc

      361 tgaaaggagg aactatatcc ggatctggcg taatagcgaa gaggcccgca ccgatcgccc

      421 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag

      481 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc

      541 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc

      601 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa

      661 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg

      721 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac

      781 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta

      841 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac

      901 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt

      961 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa

     1021 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt

     1081 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat

     1141 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag

     1201 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg

     1261 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata

     1321 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat

     1381 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc

     1441 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg

     1501 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac

     1561 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact

     1621 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa

     1681 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct

     1741 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc

     1801 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga

     1861 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac

     1921 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag

     1981 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg

     2041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc

     2101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag

     2161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt

     2221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac

     2281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc

     2341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt

     2401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt

     2461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc

     2521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt

     2581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca

     2641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt

     2701 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt

     2761 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag

     2821 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg

     2881 ccgattcatt aatgcag

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