218976 Streck游离RNA保存管血浆血清Cell-Free RNA

Streck Cell-Free RNA BCT® 血浆游离RNA保存管

产品英文名称：Streck Cell-Free RNA BCT® BLOOD COLLECTION TUBE

产品中文名称：Streck 游离RNA保存管，血浆血清

产品概述 Streck Cell-Free RNA BCT®游离RNA保存管含有一种防腐剂，它能稳定有核血细胞，防止细胞RNA的释放，是保护游离循环RNA的一种血液收集管，用于采集、运输和稳定血液游离RNA。

参数

采血量 10mL

游离RNA稳定性 18-25℃，7天

保质期 24个月

稳定性

游离RNA室温下Z高能稳定7天

没装样本的采血管保质期有24个月

应用

回复高质量的游离RNA适用于临床研究、药物开发和诊断分析发展中的靶向PCR。

概要和原理

1样本处理、运输和后期处理导致有核血细胞的细胞溶解和RNA的释放会影响cf-RNA的准确分析。此外，由于核酸酶造成RNA降解也会造成影响。

2 Streck 游离RNA保存管中的防腐剂能稳定有核血细胞,防止细胞RNA的释放和YZ核酸酶造成cf-RNA的降解，从而有利于cf-RNA和细胞RNA的稳定。

3 Streck 游离RNA保存管加强对cf-RNA和细胞RNA的纯化、检测和分析。管中的样本在18 °C - 25 °C能稳定在7天，以便于样本收集、传输和存储。

成份

包含抗凝剂K3EDTA，核酶YZ剂，代谢YZ剂和细胞防腐剂。试剂呈现红色是正常现象。

注意事项

1采集样本后不能冷冻，否则可能破裂。

2超过效期请不要使用。

3不要将注射进病人体内。

4试剂成份是固定的，不要稀释或者添加其他成份。

5样本过少或者过多都可能对Z后的分析结果造成不好的影响。

注意 a.玻璃有破碎的可能性;在处理过程中应采取预防措施。

   b.所有与它们接触的所有生物标本和材料都被认为是生物危害，按照生物安全法规处理，避免与皮肤和粘膜接触。

   c.产品应与传染性YL废物一起处置。

   d.要么通过轻轻的摇摆瓶塞或者边扭动边拉拽的方式打开瓶塞。用于瓶塞拆卸的“拇指滚动”程序不推荐，可能导致管破裂和损伤。

INSTRUCTIONS FOR USE

Cell-Free RNA BCT® is a direct-draw whole blood collection tube intended for collection, stabilization and transportation of cell-free plasma RNA. Cell-Free RNA BCT also stabilizes and preserves cellular RNA in white blood cells. This product has not been cleared by the U.S. Food and Drug Administration for In Vitro Diagnostic Use. The product is For Research Use Only. Not for use in diagnostic procedures.

SUMMARY AND PRINCIPLES

Accurate analysis of cf-RNA can be compromised by sample handling, shipping and processing, causing lysis of nucleated blood cells and subsequent release of cellular RNA. Additionally, degradation of cf-RNA due to nuclease activity can be problematic.

The preservative reagent contained in Streck’s Cell-Free RNA BCT stabilizes nucleated blood cells, preventing the release of cellular RNA, and inhibits nuclease mediated degradation of cf-RNA, contributing to the overall stabilization of both cfRNA1,2,3 and cellular RNA.4

Cell-Free RNA BCT enhances purification, detection and analysis of cf-RNA and cellular RNA. Samples collected in Cell-Free RNA BCT are stable for up to 7 days at temperatures between 18 °C to 25 °C, allowing convenient sample collection, transport and storage.

REAGENTS

Cell-Free RNA BCT contains the anticoagulant K3 EDTA, nuclease inhibitors, metabolic inhibitors and a cell preservative in a liquid medium.

Note: It is normal for the reagent in Cell-Free RNA BCT to have a reddish appearance.

STORAGE AND STABILITY

1. When stored at 18 °C to 30 °C, empty Cell-Free RNA BCT is stable through expiration date.

2. Short-term storage from 2 °C to 40 °C is acceptable for empty Cell-Free RNA BCT for up to 14 days.

3. Do not freeze unfilled Cell-Free RNA BCT. Proper insulation may be required for shipment during extreme temperature conditions.

4. Blood samples collected in Cell-Free RNA BCT are stable for up to 7 days when stored between 18 °C to 25 °。

INSTRUCTIONS FOR USE使用说明

1. Collect specimen by venipuncture according to CLSI GP41-A65.

Prevention of Backflow - Since Cell-Free RNA BCT contains chemical additives, it is important to avoid possible backflow from the tube.

To guard against backflow, observe the following precautions:

a. Keep patient’s arm in the downward position during the collection procedure.

b. Hold the tube with the stopper in the uppermost position so that the tube contents do not touch the stopper or the end of the needle during sample collection.

c. Release tourniquet once blood starts to flow in the tube, or within 2 minutes of application.

2. Follow recommendations for order of draw outlined in CLSI GP41-A65. Cell-Free RNA BCT can be drawn after the EDTA tube and before the fluoride oxalate (glycolytic inhibitor) tube. If a Cell-Free RNA BCT tube immediay follows a heparin tube in the draw order, Streck recommends collecting a non-additive or EDTA tube as a waste tube prior to collection in the Cell-Free RNA BCT.

采血管使用顺序

3. Fill tube compley.

4. Remove tube from adapter and immediay mix by gentle inversion 8 to 10 times. Inadequate or delayed mixing may result in incorrect analytical results or poor product performance. One inversion is a complete turn of the wrist, 180 degrees, and back.

5. After collection, transport and store tubes within the recommended temperature range.

Note:

1. For best results, a 21G or 22G needle is advised. Slower fill times may be observed when using a smaller gauge needle.

2. When using a winged (butterfly) collection set for venipuncture and the Streck Cell-Free RNA BCT is the first tube drawn, a non-additive or EDTA discard tube should be partially drawn first in order to eliminate air or “dead space” from the tubing.

Streck采血管订购信息

Streck Cell-Free RNA BCT® 血浆游离RNA保存管218976欢迎您咨询华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  ：1500 1904 520。红荣微再-客服:  经销商专员