V01053 必拓生物pTET载体，pTET vector，质粒载体

V01053必拓生物pTET载体，pTET vector，质粒载体图谱

来自大肠杆菌TorA蛋白的GX率信号肽，能引导外源蛋白GX率分泌，其分泌机制不同于pelB信号肽和肠毒素II的信号肽（STII），对某些蛋白的分泌效率更高。可用于外源蛋白在大肠杆菌体系中的分泌表达。载体大小为5616bp，氨苄霉素抗性。


产品特色
1.含有大肠杆菌TorA蛋白的信号肽，分泌效率高；
2.含有中等强度启动子tac启动子，表达较高；
3.载体C-末端含有His6标签序列和c-myc检测标签序列，可用于金属离子亲和层析纯化和对表达产物进行检测

成分
10 mM Tris（pH8.0）

保存条件
-20℃保存

注意事项
1.可以采用自诱导的方式分泌表达目的蛋白；
2.不同种类或不同序列的蛋白，其分泌效率差异很大，需要进行预实验确定较好的条件。

产品目录

必拓生物pTET载体，pTET vector，质粒载体图谱

LOCUS       pTET        5616 bp    DNA     circular     17-NOV-2016

FEATURES             Location/Qualifiers

     misc_feature    1119..1976

                     /vntifkey="21"

                     /label=bla(Amp)

     misc_feature    364..381

                     /vntifkey="21"

                     /label=His6

     misc_feature    complement(4171..5250)

                     /vntifkey="21"

                     /label=lacI

     misc_feature    325..357

                     /vntifkey="21"

                     /label=c-myc

     misc_feature    154..279

                     /vntifkey="21"

                     /label=SignalpeptideofE.coliTorA

     misc_feature    33..61

                     /vntifkey="21"

                     /label=tacpromoter

     rep_origin      2040..3207

                     /vntifkey="33"

                     /label=ColE1Origin

BASE COUNT     1309 a      1490 c      1493 g      1324 t

ORIGIN

        1 agatctctgg caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg

       61 tgtggaattg tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt

      121 tcacgagcac ttcaccaaca aggaccatag catatgaaca ataacgatct ctttcaggca

      181 tcacgtcggc gttttctggc acaactcggc ggcttaaccg tcgccgggat gctggggccg

      241 tcattgttaa cgccgcgacg tgcgactgcg gcgcaagcgg gatccgctag cctcgagggt

      301 accgaattcg tcgacgcggc cgcagaacaa aaactcatct cagaagagga tctgaatatc

      361 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag

      421 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc

      481 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct

      541 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg

      601 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg

      661 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac

      721 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct

      781 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt

      841 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt

      901 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt

      961 ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa

     1021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac

     1081 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg

     1141 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc

     1201 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg

     1261 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga

     1321 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc

     1381 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag

     1441 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga

     1501 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg

     1561 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga

     1621 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt

     1681 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact

     1741 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt

     1801 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg

     1861 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta

     1921 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac

     1981 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta

     2041 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt

     2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt

     2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt

     2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc

     2281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg

     2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg

     2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt

     2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac

     2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg

     2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg

     2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat

     2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt

     2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg

     2821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa

     2881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc

     2941 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg

     3001 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg

     3061 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg

     3121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca

     3181 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc

     3241 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt

     3301 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact

     3361 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag

     3421 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag

     3481 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc

     3541 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg

     3601 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca

     3661 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg

     3721 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag

     3781 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg

     3841 gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga

     3901 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag

     3961 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc

     4021 atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag

     4081 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg

     4141 agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg

     4201 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc

     4261 cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg

     4321 gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg

     4381 tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac

     4441 taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag

     4501 cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg

     4561 catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg

     4621 aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga

     4681 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac

     4741 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga

     4801 gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg

     4861 gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac

     4921 cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc

     4981 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag

     5041 actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg

     5101 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga

     5161 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc

     5221 tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg

     5281 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac

     5341 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga

     5401 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca

     5461 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc

     5521 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg

     5581 tgatgccggc cacgatgcgt ccggcgtaga ggatcg

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