白介素10(IL10)真核蛋白EPA056Mu61 100ug Eukaryotic Interleukin 10 (IL10) Organism Species: Mus musculus (Mouse) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 11th Edition (Revised in May, 2016) [ PROPERTIES ] Source: Eukaryotic expression. Host: 293F cell Residues: Ser19~Ser178 Tags: N-terminal His Tag Homology: Human 73%, Rat 85% Tissue Specificity: Spleen. Subcellular Location: Secreted. Purity: 白介素10(IL10)真核蛋白>98% Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Traits: Freeze-dried powder Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL Predicted isoelectric point: 8.2 Predicted Molecular Mass: 20.4kDa Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions. Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.) [ USAGE ] Reconstitute in 20mM Tris, 1白介素10(IL10)真核蛋白50mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, 白介素10(IL10)真核蛋白incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. [ SEQUENCE ] [ IDENTIFICATION ] Figure 1. Gene Sequencing (extract) Figure 2. 白介素10(IL10)真核蛋白SDS-PAGE
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