Ethyl methanesulfonate(EMS)甲基磺酸乙酯
描叙:
EMS ( ethyl methane sulfonate ,甲基磺酸乙酯)诱变是构建突变体库的重要方法之一.EMS 是一种烷化剂,可以诱发基因突变,不需进行遗传转化.与其他诱变剂相比,EMS 诱变产生的点突变频率高,且多为隐性突变,染色体畸变相对较少,因而被广泛地用来构建突变体库.目前,已经在哺乳动物、拟南芥、水稻、小麦等植物中利用EMS诱变技术构建了突变体库,它们在功能基因组学研究中发挥了重要作.
声明:
For Laboratory Use Only. Not for drug, household or other uses.
准备说明:
EMS is miscible in chloroform (200 mg/ml).
EMS can be diluted to a concentration of 1% in water or serum-free medium and then added to a culture at a final concentration in the range of 200-300 mg/ml.
储存/稳定性:
Reaction rate constants and half-lives for aqueous solutions of this product were calculated at increasing temperatures and at several pH's. The rate of hydrolysis is unaffected by varying (acidic) pH.
订购说明:
货号 | 名称 | 产地 | 规格 | 报价/元 | 特价/元 |
M0880 | Ethyl methanesulfonate(EMS)甲磺酸乙酯 | Sigma | 25g | 1138 | 968 |
11005ES25 | Ethyl methanesulfonate(EMS)甲磺酸乙酯 | YEASEN | 25g | 839 | 839 |
文献参考:用甲基磺酸乙酯(EMS)诱变的大麦浙农大3 号突变体的筛选和鉴定(点击下载)