Sigma L8754 5mg PHA-P,Phaseolus vulgaris agglutinin...

Analysis Note

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin （PHA-E） which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin （PHA-L） which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.^1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.³ PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.⁴ PHA-M is the mucoprotein form.⁴ Conjugates are prepared from the corresponding purified lectins.

form

lyophilized powder

potency

<62.5 μg per mL agglutination activity

<10 μg/mL mitogenic activity

total impurities

salt, free

storage temp.

2-8°C

Safety Statements

22-24/25

RTECS

TJ3470000

品名

产地

货号

规格

单价

备注

PHA-P植物血球凝聚集素

Sigma

L8754

5mg

询价

现货