辣根过氧化物酶Peroxidase
过氧化物酶，酶学分类号为EC 1.11.1.7。该酶催化
Donor+ H2O2--→Oxidized donor+2 H2O。
过氧化物酶，通常来源于辣根（因此称辣根过氧化物酶），是临床检验试剂中的常用酶。该产品不但广泛用于多个生化检测项目，也广泛运用于免疫类（ELISA）试剂盒。过氧化物酶作为多个试剂盒显色体系的关键成分，对试剂盒的质量有重要影响。
辣根过氧化物酶（Horseradish Peroxidase, HRP）比活性高，稳定，分子量小，纯酶容易制备，所以Z常用。HRP广泛分布于植物界，辣根中含量高，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18％。HRP由多个同功酶组成，分子量为40,000,等电点为PH3～9,酶催化的Z适PH因供氢体不同而稍有差异，但多在PH5左右。酶溶于水和58％以下饱和度硫酸铵溶液。HRP的辅基和酶蛋白Z大吸收光谱分别为403nm和275nm，一般以OD403nm ／OD275nm的比值RZ（德文Reinheit Zahl）表示酶的纯度。高纯度的酶RZ值应在3.0左右（Z高可达3.4）。RZ值越小，非酶蛋白就越多。
Sigma相关产品资料：
Sigma P6782 Peroxidase from horseradish
Type VI-A, essentially salt-free, lyophilized powder, ~1000 units/mg solid （using ABTS）, 250-330 units/mg solid （using pyrogallol）
| Synonym: | Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase |
| CAS Number: | 9003-99-0 |
| Enzyme Commission （EC） Number: | 1.11.1.7 （ BRENDA | IUBMB ） |
| EC Number: | 232-668-6 |
| MDL number: | MFCD00071339 |
Description
| Analysis Note | The RZ （Reinheitszahl） is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity. |
| | This product is assayed using ABTS for easy comparison to other suppliers' unit: approx. 1,000 units per mg solid |
| Linkage | Similar to P8375 |
| Other Notes | View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer. |
| Packaging | Packaged in mg solid |
| Unit Definition | One ABTS unit will oxidize 1 μmole of ABTS per minute at 25 °C at pH 5.0 |
| | One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C. |
| General description | Horseradish peroxidase is isolated from horseradish roots （Amoracia rusticana） and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight （~44 kDa） includes the polypeptide chain （33,890 Daltons）, hemin plus Ca2+ （~700 Daltons）, and carbohydrate （~9,400 Daltons）. At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0. |
性质
| type | Type VI-A |
| form | essentially salt-free, lyophilized powder |
| EQP level | Premium |
| storage temp. | 2-8°C |
安全性
| Personal Protective Equipment | Eyeshields, Gloves, type N95 （US）, type P1 （EN143） respirator filter |
| WGK Germany | 3 |
| 品名 | 产地 | 货号 | 规格 | 单价 | 备注 |
| HRP辣根过氧化物酶 | Sigma | P6782 | 10mg | 询价 | 现货 |
| HRP辣根过氧化物酶 | Sigma | P6782 | 25mg | 询价 | 现货 |
| HRP辣根过氧化物酶 | Sigma | P6782 | 50mg | 询价 | 现货 |
| HRP辣根过氧化物酶 | Sigma | P6782 | 100mg | 询价 | 现货 |
Sigma P6782 10mg Peroxidase,Horseradish peroxidase,H...
Sigma P6782 10mg Peroxidase,Horseradish peroxidase,HRP
Peroxidase (Horseradish), EIA Grade / Peroxidase (Horseradish), EIA Grade
辣根过氧化物酶(≥250u/mg),Peroxidase, Horseradish(HRP)
辣根过氧化物酶(≥300u/mg),Peroxidase, Horseradish(HRP)
辣根过氧化物酶Peroxidase Horseradish(POD/HRP)
辣根过氧化物酶价格,Peroxidase (Horseradish)
辣根过氧化物酶（POD）价格Peroxidase horseradish
辣根过氧化物酶价格Peroxidase Horseradish(POD/HRP)
辣根过氧化物酶，9003-99-0，Peroxidase, Horseradish(HRP)
Peroxidase, horseradish, Rz >2.5 辣根过氧化物酶
Peroxidase, horseradish Rz >3. 0辣根过氧化物酶
