ESG2207 Rat ESGRO®Rat LIF ESG2207

Rat ESGRO®
现货供应

Description: Rat ESGRO®

Trade Name: Chemicon (Millipore)

Product Overview: Applications for Rat ESGRO® include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF.

Key Applications: Stem Cell Culture

Application Notes: Applications for Rat ESGRO® include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama et al. 1998). 

Rat ES Cells: RtLIF produced from a transfected cell line, has been shown to effectively maintain the undifferentiated phenotype of rat ES cells when used at a concentration of 1000-1500 units / mL (Takahama et al. 1998). 

At this recommended concentration 10⁶ units of ESGRO® is sufficient for 1L of tissue culture media. 

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Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Species: Rat

Quality Assurance: The highest standards of quality control are used in the manufacture of this product. No warranty is provided that the sale or use of the product either alone, in combination with other products, or in the operation of any process, will not infringe patent, inlectual property or any other rights of third parties. This product is supplied for research purposes only. It is not for human therapeutic and/or diagnostic use.

Cell Culture Media or Reagent Type:

ESGRO Supplement

Stem Cell Type:

• Mouse Embryonic Stem Cells
• Induced Pluripotent Stem Cells

Stem Cell Workflow Stage: Maintenance & Expansion

Media Type: Cell Culture Media Additives

Source: rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.

Format: Purified

Presentation: Purified, sterile liquid. Rat ESGRO® is supplied in liquid form as 10⁷ Units ESGRO® in 1.0 mL of 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl with 1% w/v bovine serum albumin BSA as a carrier for stability. 

Rat ESGRO® is supplied 0.22 micron sterile filtered, and tested negative for mycoplasma.

Storage Conditions: Rat ESGRO® is shipped on dry ice. This product is stable for at least 12 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. 

For long term storage it is recommended that Rat ESGRO® concentrate be stored at -20°C. 

It is recommended that prior to use, Rat ESGRO® should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at -20°C. Freeze thawing will reduce potency and is not recommended.

Specific Activity: Rat ESGRO® is assessed both on murine ES {embryonic stem} cells (MBL-1) and on murine M1 myeloid leukemic cells. A standard of 50 Units is defined as the concentration of Rat ESGRO® in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies. 10⁶ units is equivalent to approximay 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media. 
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mLMurine myeloid leukemic, M1 Assay: Specific Activity > 10⁸ units/mg. 

Note: LIF should not be allowed to come in contact with ruminant or swine.

Cell Culture Reagent Type:

• Cytokines & Growth Factors
• Cell Culture Supplements

Packaging: 1 mL

Gene Symbol: NM_022196

Protein or Enzyme Type: Cytokines & Growth Factors

Alternate Names: Rat LIF

Concentration: 10 million units/1 mL

Purity: The active component rat LIF (rtLIF) has been shown to be >85% pure by SDS-PAGE.

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