血管内皮生长因子E/VEGF-E （Orf Virus）_详细资料

产品信息 VEGF E （Orf Virus） Vascular Endothelial Growth Factor-E Recombinant （Orf Virus） 血管内皮生长因子E Catalog No. CYT-263 Description: A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 （Dehio et al., 1999; GenBank accession No. AF106020） was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximay 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus （OV）（Lyttle et al., 1994）. Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins （Dehio et al., 1999; Wise et al., 1999）. Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 （KDR） resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 （Flt-1）. Furthermore VEGF-E can also not bind to VEGF receptor-3 （FLT-4）. Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR. Source: Escherichia Coli. Physical Appearance: Sterile Filtered White lyophilized （freeze-dried） powder. Formulation: The protein was lyophilized from a concentrated （1mg/ml） solution containing PBS pH-7.4. Solubility: The lyophilized VEGF-E is soluble in water and most aqueous buffers. The lyophilized Vascular Endothelial Growth Factor-E （Orf Virus） should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50µg/ml. Stability: Lyophilized Vascular Endothelial Growth Factor-E Orf Virus although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF E -OV should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. Purity: Greater than 90.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. Biological Activity: The ED₅₀ for stimulation of ³H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF-E Orf Virus has been determined to be in the range of 5 - 20 ng/ml, corresponding to a specific activity of 2 x 10⁵Units/mg. Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 相关产品： VEGF 重组人血管内皮生长因子 CYT-241 Recombinant Human Vascular Endothelial Growth Factor VEGF His 重组人血管内皮生长因子 His CYT-496 Recombinant Human Vascular Endothelial Growth Factor His Tag VEGF CHO 重组人血管内皮生长因子 CHO CYT-260 Recombinant Human Vascular Endothelial Growth Factor, CHO VEGF Sf9 重组人血管内皮生长因子 Sf9 CYT-225 Recombinant Human Vascular Endothelial Growth Factor, Sf9 VEGF Yeast 重组人血管内皮生长因子 Yeast CYT-577 Recombinant Human Vascular Endothelial Growth Factor, Yeast VEGF （121a.a.）重组人血管内皮生长因子（121a.a.） CYT-343 Recombinant Human Vascular Endothelial Growth Factor （121） VEGF （121a.a.） Sf9 重组人血管内皮生长因子（121a.a.） Sf9 CYT-200 Recombinant Human Vascular Endothelial Growth Factor （121）, Sf9 VEGF （121a.a.） His 重组人血管内皮生长因子（121a.a.） His CYT-619 Recombinant Human Vascular Endothelial Growth Factor （121） His Tag rmVEGF 重组小鼠血管内皮生长因子 CYT-336 Recombinant Mouse Vascular Endothelial Growth Factor mVEGF Sf9 重组小鼠血管内皮生长因子 Sf9 CYT-226 Recombinant Mouse Vascular Endothelial Growth Factor-Sf9 rmVEGF （121a.a.）重组小鼠血管内皮生长因子（121a.a.） CYT-574 Recombinant Mouse Vascular Endothelial Growth Factor （121） rVEGF 重组大鼠血管内皮生长因子 CYT-392 Recombinant Rat Vascular Endothelial Growth Factor mVEGF His 重组小鼠血管内皮生长因子 His CYT-680 Recombinant Mouse Vascular Endothelial Growth Factor His Tag VEGFB 重组人血管内皮生长因子B CYT-628 Recombinant Human Vascular Endothelial Growth Factor B VEGF-C 重组人血管内皮生长因子相关蛋白 CYT-527 Recombinant Human Vascular Endothelial Growth Factor Related Protein rVEGF-C 重组大鼠血管内皮生长因子相关蛋白 CYT-262 Recombinant Rat Vascular Endothelial Growth Factor Related Protein rVEGF-C （152a.a.）重组大鼠血管内皮生长因子C（152a.a.） CYT-284 Recombinant Rat Vascular Endothelial Growth Factor-C （152） VEGF-E （Orf Virus）重组血管内皮生长因子E（Orf Virus） CYT-263 Recombinant Vascular Endothelial Growth Factor-E （Orf Virus） VEGI 重组人血管内皮细胞生长YZ剂 CYT-517 Recombinant Human Vascular Endothelial Growth Inhibitor Visfatin 重组人内脂素 CYT-318 Recombinant Human Visfatin Visfatin His 重组人内脂素 His CYT-563 Recombinant Human Visfatin, His Tag mVisfatin 重组小鼠内脂素 CYT-447 Recombinant Mouse Visfatin

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