目录号 | 产品名称 | 中文名称 | 规格 | 价格 |
1051 | Cy 3 acid, mono-NHS ester | Cy 3-N-羟基琥珀酰亚胺酯 | 1 mg | 2050.00 |
分子式:C35H40KN3O10S2;分子量:765.95;Ex:548nm,Em:562nm;用于标记蛋白、多肽及抗体,溶于DMF及DMSO;−20°C干燥避光保存
Cy3 NHS Ester
Structure
MW = 765.95
Structure shown as mono-functional NHS ester.
Spectra
Excitation and emission spectra of unconjugated Cy3 dye in phosphate buffered saline.
Properties | |
Excitation maximum (nm) | 548 |
Emission maximum (nm) | 562 |
Extinction coefficient (M-1 cm-1) | 150 000 * |
Quantum yield | 0.04 * |
Fluorescence lifetime (ns) | <0.3 |
* Measured in phosphate-buffered saline, Mujumdar, R. B., Ernst, L. A., Mujumdar, S. R., Lewis, C. J., and Waggoner, A. S. (1993) Bioconjugate Chem. 4, 105-111.
(Note: The quantum yield of Cy3 is significantly increased when in a rigid microenvironment, for example in highly viscous solvents, or when conjugated to biomolecules).
Upon attachment to biomolecules (for example oligonucleotides), negligible shifts in excitation and emission wavelength have been observed. In addition no effects on these spectral characteristics have been observed in the presence of 10% DMSO or under a range of pH conditions.