26901 Anti-Active Gαi Mouse Monoclonal An...

Active Gαi
Catalog Number: 26901
Gene Symbol: Gnai
Description: Anti-Active Gαi Mouse Monoclonal Antibody

Background: Heterotrimeric G proteins are critical
cellular signal transducers. Gαi represents one
sub-family of G proteins that could mediate the
inhibition of adenylyl cyclases. Other biochemical
and physiological functions of Gαi proteins are
being explored.
Immunogen: Recombinant full length protein of
active Gαi1
Tested applications: IP, IHC
Recommended dilutions:
1 μg for 1~2 mg total cellular proteins
Concentration: 1 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS （without Mg2+ and Ca2+）, pH 7.4,
150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Gαi antibody
recognizes active Gαi1, Gαi2, and Gαi3 of
vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze /
thaw cycles

Gαi activation assay. A. CHO cells were transfected
with wild-type Gαi1 （lanes 1 and 2） or constitutively active
Gαi1-Q204L （lane 3）. Cell lysates were treated with GDP
（lane 1） or GTPγS （lane 3）. Lysates were then incubated
with an anti-active Gαi monoclonal antibody （Cat. No.
26901） （top panel）. The precipitated active Gαi was
immunoblotted with an anti- Gαi monoclonal antibody （Cat.
No. 26003）. The bottom panel shows the Western blot with
anti- Gαi monoclonal antibody （Cat.No. 26003） of the cell
lysates. B. HEK293 cells stably expressing human m2
mAChR were treated with （lane 2） or without （lane 1）
carbachol. Cell lysates were then incubated with an
anti-active Gαi monoclonal antibody （Cat. No. 26901） （top
panel）. The precipitated active Gαi was immunoblotted
with an anti- Gαi rabbit polyclonal antibody （Cat. No.
21006）. The bottom panel shows the Western blot with
anti-tubulin of the cell lysates..