Monoclonal Anti cGMP EIA Kit

Monoclonal Anti-cGMP Antibody Based
Direct cGMP ELISA Kit
（New Non-acetylated Version）
Catalog No. 80103
96 Well Kit

Product Description
Guanosine 3', 5'-cyclic monophosphate （cyclic GMP; cGMP） plays
critical regulatory roles in many physiological processes. cGMP is
produced from GTP by guanylyl cyclases and is degraded by
phosphodiesterases. Stimulation of guanylyl cyclases or inhibition of
phosphodiesterases can increase cellular cGMP concentrations.
Inhibitors of the cGMP-specific
phosphodiesterases are used for
treating human diseases. For
example, inhibitors of cGMP specific
phosphodiesterase type 5 （such as
Viagra and Cialis） are used for
treating erectile dysfunction.
To screen for inhibitors of
phosphodiesterases or stimulators of
guanylyl cyclases, it is essential to
have a fast, sensitive, selective and
reproducible method to measure the
cGMP concentrations. This is
especially true for the initial
screenings given the possible weaker
effects of larger pools of compounds.
Currently available other ELISA kits measuring cGMP levels are based
on the non-affinity-purified polyclonal anti-cGMP antibody. Despite the
claimed selectivity, these polyclonal anti-cGMP antibodies display
certain cross-reactivity with cAMP or GTP. In most cell types, cGMP is
present at levels ~100 fold lower than cAMP.

NewEast Biosciences cGMP ELISA kit is based on the unique mouse
monoclonal anti-cGMP antibody. This monoclonal anti-cGMP
antibody displays >108 fold of selectivity over cAMP, GTP, and other
nucleoside analogues. NewEast Biosciences cGMP ELISA kit provides
significantly improved sensitivity and selectivity over other kits based
on polyclonal anti-cGMP antibodies. Our monoclonal anti-cGMP
antibody-based ELISA kit also avoids the batch-to-batch variations
associated with polyclonal antibody productions from animals, thus
providing the reproducibility in the long run.
Furthermore, while polyclonal anti-cGMP antibodies used in other
ELISA kits have higher affinity for acetylated cGMP than non-acetylated
cGMP, NewEast Biosciences monoclonal anti-cGMP antibody has
similar affinities to non-acetylated and acetylated cGMP molecules.
Therefore, acetylation treatments of samples and standards are not needed
in NewEast Biosciences cGMP ELISA kit. This significantly reduces
the time for the assay. The avoidance of organic reagents used in the
acetylation process provides a safe and healthy work environment.