FuGENE® 6 Transfection Reagent
名称 | 货号 | 规格 | 价格 | 反应次数 |
FuGENE 6 Transfection Reagent | 11815091001 | 0.4 ml | 1764 | 120 |
11814443001 | 1 ml | 3545 | 300 |
| 10 ml | 37652 | 3000 |
11988387001 | 5 x 1 ml | 15935 | 1500 |
产品应用:
FuGENE® 6 Transfection Reagent is a non-liposomal multicomponent reagent that is perfect for experiments involving cellular analysis. Its characteristics (listed under "Benefits" below) make it ideal for transfection of a wide range of cells.
Note: To see a list of more than 700 types of cells that have been successfully transfected with FuGENE® 6 Transfection Reagent, including many "hard-to-transfect" cell types, visit our Transfection Special Interest Site and view our Transfection Reference Database. This site also contains much more information about the use of this reagent.
For life science research only.
产品优势:
Works on a broad range of cells, including many "hard-to-transfect" cells.
More likely to generate results that are physiologically relevant, since it has very low cytotoxicity and does not disturb cell mechanisms. A recent gene-chip study (Figure 1) demonstrated that transfection reagent from a competitor significantly influenced the expression of more than 2,000 genes, while FuGENE® 6 Transfection Reagent influenced only 30 genes.
Figure 1: Alterations in gene expression following transfection with FuGENE® 6 Transfection Reagent or Reagent L. The number of genes affected after transfection with FuGENE® 6 Transfection Reagent (c) is compared with those affected after transfection with reagent L (d). Changes in the genes expressed by the plasmid without insert (a) and the plasmid coding for secreted human placental alkaline phosphatase (SEAP) (b) were compared after transfection with the two transfection reagents. Overlapping areas represent genes with altered expression levels on all the plasmids (c and d) or with both transfection reagents (a and b).
Allows you to transfect in serum-free or serum-containing media. FuGENE® 6 Transfection Reagent is highly efficient both in the presence and absence of serum (Figure 2). In most cases, the same cell culture medium used for routine cell culture (e.g., one that contains serum) can also be used during transfection. This is especially useful in cases where transfection efficiency improves in the presence of serum.
Important note: The FuGENE® 6 Transfection Reagent and plasmid complex can be added directly to cells that are cultivated in complete medium with FCS and other additives. However, serum-free medium without any additives must be used for dilution of FuGENE® 6 Transfection Reagent and the formation of the complex.
Product Description
Composition: contains a proprietary blend of lipids and other components in 80% ethanol.
Sterility: filter-sterilized.
背景信息:
Mentioned in over 8,500 publications, FuGENE® 6 Transfection Reagent is one of the most widely used transfection reagents in the world.
Contents
Lipid blend in 80% ethanol, sterile-filtered, packaged in glass vials.