500ML- BCA Protein Assay Reagent,BCA蛋白定量试剂盒

BCA蛋白定量试剂盒中文名：BCA (tm) Protein Assay Kit英文名：BCA蛋白定量试剂盒Pierce相关资料如下：BCA Protein Assay Reagent (bicinchoninic acid) 

Used in more labs than any other detergent-compatible protein assay. The Thermo Scientific Pierce BCA Protein Assay remains one of the most popular protein assay methods worldwide. The detergent-compatible method provides for accurate determination of protein concentration with most sample types encountered in protein research laboratories. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity. Highlights: Colorimetric method; read at 562 nm Compatible with most ionic and nonionic detergents Faster and easier than the Lowry method Working reagent stable for 24 hours Linear working range for BSA from 20-2,000 µg/ml Minimum detection level of 5 µg/ml with the enhanced protocol Adaptable to microplates Less protein-to-protein variation than dye-binding methods BCA Protein Assay Applications: Studying protein:protein interactions Measuring column fractions after affinity chromatography Estimating percent recovery of membrane proteins from cell extracts High-throughput screening of fusion proteins Substances compatible with the Thermo Scientific BCA Protein Assay. The following is a short list of compatible substances and their concentrations that the BCA Protein Assay can tolerate. For a more complete list please refer to the instruction booklet for the product or view our Table of Compatible Substances for a comparison of substance compatibilities among several different protein assay methods. Substance Compatible Conc. Substance Compatible Conc. NP-40 5.0% Glucose (fresh) 10 mM Emulgen 1.0% EDTA 10 mM Hepes 100 mM Sodium Chloride 1.0 M DTT 1 mM NaOH 0.1 M Guanidine•HCl 4.0 M Ammonium Sulfate 1.5 M Triton X-100 5.0% Sodium Acetate, pH 5.5 200 mM Octyl-ß-Glucoside 5.0% SDS 5.0% Urea 3.0 M Brij-35 5.0% Sucrose (fresh) 40% Lubrol 1.0% Glycine, pH 2.8 100 mM CHAPS 5.0% Please refer to the product instructions for special notes and definitions about compatibility tolerances.   Time comparison of BCA Protein Assay Reagent vs. Lowry Protein Assay. BCA Protein Assay Reagent 1. Mix reagents 1 minute 2. Add sample and incubate 30 minutes 3. Read at 562 nm 1 minute Total BCA Time: 32 minutes Lowry Protein Assay 1. Make reagents 70 minutes 2. Add sample and incubate 20 minutes 3. Add Folin Reagent 1 minute 4. Incubate 30 minutes 5. Read at 750 nm 1 minute Total Lowry Time: 122 minutes   您可能感兴趣的产品 500ML- BCA Protein Assay Reagent,BCA蛋白定量试剂盒 货号A6932 Bradford蛋白定量溶液 货号A6932 Bradford蛋白定量溶液 蛋白定量 C0088 总蛋白定量测试盒（带标准，考马斯亮兰法） C0088-1 总蛋白定量测试盒（带标准，双缩脲法） C0121 尿蛋白定量测试盒 总蛋白定量测试盒（带标准，考马斯亮兰法） 总蛋白定量测试盒（带标准，双缩脲法） 尿蛋白定量测试盒 总蛋白定量测试盒（带标准，考马斯亮兰法） C0088 总蛋白定量测试盒（带标准，双缩脲法） C0088-1 相关产品 供应商产品推荐 Invitrogen 18374058 10次- 5´RACE System for Rapid Amplif... 了解详情 100mg- 5-氮杂胞苷,5-azacytidine,Ladakamycin,Az... 了解详情 25MG- bisBenzimide H 33342,HOE 33342, Hoe... 了解详情 500ML- BCA Protein Assay Reagent,BCA蛋白定量试剂... 了解详情 Invitrogen 12578035- GB C-TERM GATEWAY IN VITRO KIT 了解详情 信息说明 本产品信息由（上海叶舟生物科技有限公司）为您提供，内容包括（ 500ML- BCA Protein Assay Reagent,BCA蛋白定量试剂盒）的品牌、型号、技术参数、详细介绍等；如果您想了解更多关于（ 500ML- BCA Protein Assay Reagent,BCA蛋白定量试剂盒）的信息，请直接联系供应商，给供应商留言。若当前页面内容侵犯到您的权益，请及时告知我们，我们将马上修改或删除。 关于我们 | 诚聘英才 | 客户投诉 | 本站服务 | 会员中心 | 买家中心 仪器网(www.yiqi.com) --专业分析仪器,检测仪器平台,实验室仪器设备交易网,仪器行业专业网络宣传推广媒体。 仪器网(www.yiqi.com)--仪器拼音域名网址,简单,易懂,好记,可信; 符合仪器用户的记忆和使用习惯,是仪器选型采购的专业平台。 销售电话: 400 822 6768 企业销售咨询: 021-68559399 021-68551566 VIP会员售后QQ: 83550177 VIP会员销售QQ: 65884322 网站售后: 021-50353779 综合业务QQ: 11694366 免费企业会员售后QQ: 932545765 展会合作QQ: 784540580 仪器求购QQ: 61224355 Copyright 2004-2023 www.yiqi.com All Rights Reserved. 沪ICP备16012899号 沪B220170422号 互联网药品信息服务资格证书 沪公网安备 31011502008050号 若仪器网内容侵犯到您的权益，请及时告诉我们,我们马上修改或删除。邮箱：hezou_yiqi X您尚未登录 会员登录没有账号？免费注册 邮  箱： 密  码： 验证码： 换一张？  下次自动登录忘记密码？ 在线留言 官方微信 仪器网微信公众号 扫码获取最新信息 在线客服 咨询客服 在线客服 工作日:  9:00-18:00 联系客服 企业专属客服 电话客服:  400-822-6768 工作日:  9:00-18:00 搜一搜 仪器社区 关注的企业 发求购 订阅商机 仪采招微信公众号 采购信息一键获取海量商机轻松掌控