500ML- BCA Protein Assay Reagent,BCA蛋白定量试剂盒

Used in more labs than any other detergent-compatible protein assay.

The Thermo Scientific Pierce BCA Protein Assay remains one of the most popular protein assay methods worldwide. The detergent-compatible method provides for accurate determination of protein concentration with most sample types encountered in protein research laboratories. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Highlights:

• Colorimetric method; read at 562 nm
• Compatible with most ionic and nonionic detergents
• Faster and easier than the Lowry method
• Working reagent stable for 24 hours
• Linear working range for BSA from 20-2,000 µg/ml
• Minimum detection level of 5 µg/ml with the enhanced protocol
• Adaptable to microplates
• Less protein-to-protein variation than dye-binding methods

  BCA Protein Assay Applications:

  • Studying protein:protein interactions
  • Measuring column fractions after affinity chromatography
  • Estimating percent recovery of membrane proteins from cell extracts
  • High-throughput screening of fusion proteins

    Substances compatible with the Thermo Scientific BCA Protein Assay. The following is a short list of compatible substances and their concentrations that the BCA Protein Assay can tolerate. For a more complete list please refer to the instruction booklet for the product or view our Table of Compatible Substances for a comparison of substance compatibilities among several different protein assay methods.
    Substance	Compatible Conc.	Substance	Compatible Conc.
    NP-40	5.0%	Glucose (fresh)	10 mM
    Emulgen	1.0%	EDTA	10 mM
    Hepes	100 mM	Sodium Chloride	1.0 M
    DTT	1 mM	NaOH	0.1 M
    Guanidine•HCl	4.0 M	Ammonium Sulfate	1.5 M
    Triton X-100	5.0%	Sodium Acetate, pH 5.5	200 mM
    Octyl-ß-Glucoside	5.0%	SDS	5.0%
    Urea	3.0 M	Brij-35	5.0%
    Sucrose (fresh)	40%	Lubrol	1.0%
    Glycine, pH 2.8	100 mM	CHAPS	5.0%
    Please refer to the product instructions for special notes and definitions about compatibility tolerances.

     

    Time comparison of BCA Protein Assay Reagent vs. Lowry Protein Assay.
    BCA Protein Assay Reagent 1. Mix reagents 1 minute 2. Add sample and incubate 30 minutes 3. Read at 562 nm 1 minute Total BCA Time: 32 minutes	Lowry Protein Assay 1. Make reagents 70 minutes 2. Add sample and incubate 20 minutes 3. Add Folin Reagent 1 minute 4. Incubate 30 minutes 5. Read at 750 nm 1 minute Total Lowry Time: 122 minutes

    
    
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