Thermo RevertAid Master Mix/Fermentas K1622反转录试剂盒升级款 M1631/M1632

描述Thermo Scientific RevertAid Master Mix is a complete system for efficient synthesis of first-strand cDNA from mRNA or total RNA templates. The mix includes RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. RevertAid Master Mix provides all cDNA synthesis reaction components in a convenient two-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications. The two-tube formulation has a shorter reaction time and requires fewer pipetting steps than the RevertAid RT Reverse Transcription Kit, while maintaining high performance.

Highlights
• Fast—short reaction time (typically 30–60 min)
• Convenient—easy workflow with few pipetting steps
• Reliable—reverse transcription of both abundant and limited targets
• Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse-transcription reaction

Applications
First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis
 规格

Reverse Transcriptase:	ThermoScript™
Downstream Application:	PCR, Real-Time PCR
Reaction Format:	SuperMix or Master Mix
Sample Type (General):	Cells
Product Line:	RevertAid
Product Size:	100 reactions
Shipping Condition:	Dry Ice

内容及储存• 2 x 50 µL of 20X Enzyme mix (MuLV and RNase inhibitor protein)
• 2 x 500 µL of 2X RT Buffer Mix (includes dNTPs, random octamers, and Oligo dT-16)

Store at -15 to -25°C. 2X RT Buffer Mix can be stored at 2–8°C for up to 6 months.