货号： PGmatrix
PGmatrix™ is a powerful and yet easy-to-use tool for in-vitro 3D cell culture with high-throughput screening and accurate in-vivo prediction for life science research and development. PGmatrix is formulated from peptide solution, and is offered pre-mixed with basic cell culture medium (DMEM, MEM, RPMI, L15), or in custom formulas.
PGmatrix is incredibly easy to use, and allows you to get accurate results faster. Unlike traditional 3D cell culture systems, PGmatrix requires no complicated steps for preparation, no pre-chilled gel solutions and no toxic cross-linking reagents.
| No acidic or chill pre-gel solution | Mimics cell microenvironment |
| Cells are easily encapsulated | Fast hydrogel formation (5-30 min) |
| Purified Synthetic Peptide in neutral pH | Works with standard methodologies |
| Cell suspension at room temperature | Easy for cell harvest after 3D culture |
A regular PGmatrix research kit includes 6mL 1% W/V PGmatrix nanofiber solution and 2mL PGworks solution. Most cells grow well in PGmatrix concentration from 0.2-0.5% W/V).
PGmatrix descriptions and usesPGmatrix has been used by researchers to create many different types of cell lines. The list below should help you decide what is the best product to order:
| Product | Cell Lines tested in PepGel in 3D |
|---|---|
| PGmatrix MEM | Breast cancer cell MCF-7 |
| PGmatrix L-15 | Breast cancer cells MDA-MB-231 & MDA-MB-468 |
| PGmatrix DMEM | Head and neck squamous cell carcinoma HN5, Ham’ F12 skin cell, HeLa cancer cell Liver cells Endothelial cells Islet cells Mouse Stem cells Pig stem cells human fibroblast cell lines, human glioblastoma stem like cells T-cells - Jurkat cells, CHO-S |
| PGmatrix RPMI | ZR-75 Prostate cancer cell (LNCaP) Islet cells, Lymphoma cells |
| PGmatrix DMEM* GFs | hiPSC derived from human fibroblast MSCs |
| PGmatrix Customized | Human ESCs, iPSCs, MSCs |
PGmatrix has been used successfully in the creation of cell lines from cancer cells and stem cells. Click on the images below to read about recent success stories.
ZR-75 | MCF-7 |
Researchers have used PGmatrix in the creation of other cell lines, including MDA-MB-231 and MDA-MB-468 human breast cancer cells, Head and neck squamous cell carcinoma HN5, Rat embryonic stem cell (ESC), and Rat induced pluripotent stem cell (iPSC).
In addition, recently PGmatrix has been successfully used in cell lines for stem cells (MSC), skin cells (Ham’s F12), prostate cancer cells (LNCaP), and HeLa cancer cells.
Other relevant publications include:
3D-printing enabled micro-assembly of a microfluidic electroporation system for 3D tissue engineering.
Lab Chip, 2019, 19, 2362.
3D cell culture stimulates the secretion of in vivo like exosomes.
Scientific ReportS | (2019) 9:13012.
hiPSC-qualified PGmatrix™ – case study.
Liang J, Liu G, Wang J, Sun XS Controlled release of BSA-linked cisplatin through a PepGel self-assembling peptide nanofiber hydrogel scaffold. Amino Acids: The Forum for Amino Acid, Peptide and Protein Research, 29 May 2017.
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PGmatrix™ 3D cell culture
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3-D Culture Cell Loading Buffers
3-D Culture Cell Harvest Buffers
3-D Culture Cell Harvest Buffers
3D Culture Cell Harvesting Kit (20 Tests)
3-D Culture Cell Loading Buffers (10 mL)
3-D Culture Cell Harvest Buffers (100 mL)
人培养细胞基因组DNA-Human Culture Cell Genomic DNA
Medum A for GIP secreting primary culture cell
Ligand 1 for GLP-1 secreting primary culture cell: Linoleic acid
Assay plate for GLP-1 secreting primary culture cell(384well)
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