Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
Recognition sites | TGC↑GCA |
来源 | Acinetobacter calcoaceticus 16 |
Assayed on | Lambda DNA |
Optimal buffer | W?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.) |
Optimal temperature | 37℃ |
Storage conditions | 10mMTris-HCl(pH 7.5);200 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20 |
Ligations | After 5-fold overdigestion with enzyme about 90%of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃. |
Methlation effect | Methylation sensitivity not tested |
Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
Campatible Ends | AviII,FspI,MstI,NsbI |