Invitrogen™ChargeSwitch™ Forensic DNA Purification Kit
货号: CS11200
相关应用: DNA 纯化
| Forensics
规格
Sample Type (General): | Forensic Samples |
Isolation Technology: | ChargeSwitch® Chemistry |
Downstream Application: | Forensic Testing, PCR |
High Throughput Compatibility: | High Throughput-Compatible |
Product Line: | ChargeSwitch® |
Product Size: | 100 preps |
Shipping Condition: | Room Temperature |
内容及储存All components of the ChargeSwitch® Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4“C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.
Components:ChargeSwitch® Lysis Buffer (L13)
ChargeSwitch® Magnetic Beads
Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
ChargeSwitch® Purification Buffer (N5)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)描述ChargeSwitch® Forensic DNA Purification Kit uses magnetic bead technology to allow for rapid and efficient purification of genomic DNA from a variety of forensic samples, including poor or environmentally degraded samples, and is fully validated for Single Tandem Repeat (STR) analysis.
• Optimized for higher-purity DNA extraction
• Validated for STR profiling on diverse forensic samples-set
• Compatible with high-throughput protocols
Rapid, Three Step, pH Based Purification ProtocolChargeSwitch® Forensic DNA Purification Kit allows purification of genomic DNA typically in less than 15 minutes following sample preparation and lysis, using magnetic beads with a unique, ionizable, nucleic acid-binding ligand whose charge can be switched based on the pH of the surrounding medium. Purification is achieved through a simple three-step procedure in aqueous buffers and avoids the use of guanidine, ethanol, and other troublesome reagents.
Validated for Single Tandem Repeat (STR) ProfilingChargeSwitch® Forensic DNA Purification Kit is suitable for use in STR analysis, for mitochondrial DNA (mtDNA) extraction from hair follicles for single nucleotide polymorphism (SNP) analysis, and includes a flexible protocol to facilitate genomic DNA isolation from a variety of forensic sample types (Figure 1), such as:
• Dried blood spots on paper and clothing
• Hair follicles and hair shafts
• Cigarette butts
• Cigarette paper
• Envelopes
• Drinking vessel swabs
• Chewing gum
• Sperm head cells
• Vaginal epithelial cells
• Door handle swabs
• Strip removed cells (e.g., hats, coats, and gloves)
• "Touch DNA" (e.g., tools, mobile phones, and microscopes)
• Dyed denim
Compatible with Single Tube and High-Throughput ProtocolsChargeSwitch® Forensic DNA Purification Kit is designed for automated processing of both small numbers of samples individually, as well as large numbers of samples using a liquid handling robot or a multi-channel pipette.
For processing up to 24 individual samples at a time the MagnaRack™ two-piece magnetic separation tube rack will be required. This rack consists of a magnetic base station and a removable tube rack, which can hold up to 24 microcentrifuge tubes and can be used with most ChargeSwitch® products.
For high-throughput applications utilizing 96 well blocks, the 96-Well Magnetic Separator will be required. This rack can hold and separate up to 96 magnetic bead samples in a deep well plate when placed on top of the magnetic base station.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
‹ 收起For Research Use Only. Not for use in diagnostic procedures.图表
Figure 1 - ChargeSwitch® Forensic DNA Purification Kit effectively purifies DNA from challenging forensic samples
温馨提示:不可用于临床ZL。
资料下载:
chargeswitch_forensicdna_man.pdf
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