测序及更多信息: 采购地址:淘宝店铺地址 我们的官网 http://hedgehogbio.comhttp://hedgehogbio.com/ pCasSA (Plasmid #98211) 载体作用 CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus 载体骨架 pCas9,pCRISPR,pKOR1 构建方法
载体类型 金黄色葡萄球菌Bacterial Expression, CRISPR, Synthetic Biology 载体抗性 Kan 筛选标记 Chloramphenicol (in S. aureus) 备注 The plasmid causes the E. coli to grow very slowly at 30C and plates may need to be incubated for 24-36 hours in order to see colonies
参考文献 Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System. Chen W, Zhang Y, Yeo WS, Bae T, Ji Q. J Am Chem Soc. 2017 Mar 15;139(10):3790-3795. doi: 10.1021/jacs.6b13317. Epub 2017 Mar 2. 10.1021/jacs.6b13317 PubMed 28218837