Human Troponin C, slow skeletal and cardiac muscles, TNNC1 ELISA

Human Troponin C, slow skeletal and cardiac muscles, TNNC1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TNNC1,CMD1Z; CMH13; TN-C; TNC; TNNC; cardiac troponin C; slow twitch skeletal/cardiac muscle troponin C; troponin C type 1 (slow); troponin C1, slow; troponin C1, slow skeletal and cardiac type

Search name

Human TNNC1 ELISA Kit ,Human CMD1Z ELISA Kit ,Human CMH13 ELISA Kit ,Human TN-C ELISA Kit ,Human TNC ELISA Kit ,Human TNNC ELISA Kit ,Human cardiac troponin C ELISA Kit ,Human slow twitch skeletal/cardiac muscle troponin C ELISA Kit ,Human troponin C type 1 (slow) ELISA Kit ,Human troponin C1 slow ELISA Kit ,Human troponin C1 slow skeletal and cardiac type ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Troponin C, slow skeletal and cardiac muscles, TNNC1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Troponin C,slow skeletal and cardiac muscles,TNNC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Troponin C,slow skeletal and cardiac muscles,TNNC1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Troponin C,slow skeletal and cardiac muscles,TNNC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Troponin C,slow skeletal and cardiac muscles,TNNC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.