Mouse Troponin C, slow skeletal and cardiac muscles, TNNC1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TNNC1,CMD1Z; CMH13; TN-C; TNC; TNNC; cardiac troponin C; slow twitch skeletal/cardiac muscle troponin C; troponin C type 1 (slow); troponin C1, slow; troponin C1, slow skeletal and cardiac type
Search name
Mouse TNNC1 ELISA Kit ,Mouse CMD1Z ELISA Kit ,Mouse CMH13 ELISA Kit ,Mouse TN-C ELISA Kit ,Mouse TNC ELISA Kit ,Mouse TNNC ELISA Kit ,Mouse cardiac troponin C ELISA Kit ,Mouse slow twitch skeletal/cardiac muscle troponin C ELISA Kit ,Mouse troponin C type 1 (slow) ELISA Kit ,Mouse troponin C1 slow ELISA Kit ,Mouse troponin C1 slow skeletal and cardiac type ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Troponin C, slow skeletal and cardiac muscles, TNNC1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Troponin C,slow skeletal and cardiac muscles,TNNC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse Troponin C,slow skeletal and cardiac muscles,TNNC1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Troponin C,slow skeletal and cardiac muscles,TNNC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Troponin C,slow skeletal and cardiac muscles,TNNC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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