Human 2- aminoethanethiol dioxygenase, ADO ELISA KIT

Product Name:Human 2- aminoethanethiol dioxygenase, ADO ELISA KIT
Packing:96T

Catalog No.:ELI-34823h

Gene Name:ADO

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:2-aminoethanethiol dioxygenase(ADO

Alternative Name:ADO,2-aminoethanethiol dioxygenase(ADO
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human 2- aminoethanethiol dioxygenase, ADO ELISA KIT allows for the in vitro quantitative determination of 2-aminoethanethiol dioxygenase(ADO concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human 2- aminoethanethiol dioxygenase, ADO ELISA KIT has been pre-coated with an 2-aminoethanethiol dioxygenase(ADO antibody specific to 2-aminoethanethiol dioxygenase(ADO .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 2-aminoethanethiol dioxygenase(ADO and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 2-aminoethanethiol dioxygenase(ADO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of 2-aminoethanethiol dioxygenase(ADO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!